| Autor: |
Kang SY; Dietetics Team, Cheju Halla General Hospital, Jeju 63127, Korea. ksi3379@naver.com., Kim E; Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea. sky90710@naver.com., Kang I; Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea. inhaek@jejunu.ac.kr., Lee M; Department of Food and Nutrition, Research Institute of Obesity Sciences, Sungshin Women's University, Seoul 01133, Korea. mlee@sungshin.ac.kr., Lee Y; Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea. lyk1230@jejunu.ac.kr. |
| Abstrakt: |
Laminaria japonica ( LJ ) and Hizikia fusiforme ( HF ) are brown seaweeds known to have various health-promoting effects. In this study, we investigated the anti-diabetic effects and possible mechanism(s) of LJ and HF by using both in vitro and in vivo models. C2C12 myotubes, mouse-derived skeletal muscle cells, treated with LF or HF extracts were used for the in vitro model, and muscle tissues from C57BL/6N mice fed a high-fat diet supplemented with 5% LF or HF for 16 weeks were used for the in vivo model. Although both the LF and HF extracts significantly inhibited α-glucosidase activity in a dose-dependent manner, the HF extract had a superior α-glucosidase inhibition than the LF extract. In addition, glucose uptake was significantly increased by LJ - and HF -treated groups when compared to the control group. Phosphorylation of protein kinase B and AMP-activated protein kinase was induced by LJ and HF in both the vivo and in vitro skeletal muscle models. Furthermore, LJ and HF significantly decreased tumor necrosis factor-α whereas both extracts increased interleukin (IL)-6 and IL-10 production in lipopolysaccharide-stimulated C2C12 myotubes. Taken together, these findings imply that the brown seaweeds LJ and HF could be useful therapeutic agents to attenuate muscle insulin resistance due to diet-induced obesity and its associated inflammation. |
