Coacervation-Mediated Combinatorial Synthesis of Biomatrices for Stem Cell Culture and Directed Differentiation.

Autor:	Wieduwild R; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Wetzel R; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany.; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstraße 105, 01307, Dresden, Germany., Husman D; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Bauer S; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., El-Sayed I; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Duin S; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Murawala P; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Thomas AK; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany., Wobus M; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstraße 105, 01307, Dresden, Germany., Bornhäuser M; Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstraße 105, 01307, Dresden, Germany.; University Hospital Carl Gustav Carus der Technischen Universität Dresden, Medizinische Klinik und Poliklinik I, Fetscherstraße 74, 01307, Dresden, Germany., Zhang Y; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, Arnoldstraße 18, 01307, Dresden, Germany.
Jazyk:	angličtina
Zdroj:	Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2018 May; Vol. 30 (22), pp. e1706100. Date of Electronic Publication: 2018 Apr 16.
DOI:	10.1002/adma.201706100
Abstrakt:	Combinatorial screening represents a promising strategy to discover biomaterials for tailored cell culture applications. Although libraries incorporating different biochemical cues have been investigated, few simultaneously recapitulate relevant biochemical, physical, and dynamic features of the extracellular matrix (ECM). Here, a noncovalent system based on liquid-liquid phase separation (coacervation) and gelation mediated by glycosaminoglycan (GAG)-peptide interactions is reported. Multiple biomaterial libraries are generated using combinations of sulfated glycosaminoglycans and poly(ethylene glycol)-conjugated peptides. Screening these biomaterials reveals preferred biomatrices for the attachment of six cell types, including primary mesenchymal stromal cells (MSCs) and primary neural precursor cells (NPCs). Incorporation of GAGs sustains the expansion of all tested cell types comparable to standard cell culture surfaces, while osteogenic differentiation of MSC and neuronal differentiation of NPC are promoted on chondroitin and heparan biomatrices, respectively. The presented noncovalent system provides a powerful tool for developing tissue-specific ECM mimics. (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze:	MEDLINE
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