Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2 T , the second Intestinimonas species cultured from the human gut.

Autor: Afouda P; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Durand GA; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Lagier JC; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Labas N; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Cadoret F; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Armstrong N; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Raoult D; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France., Dubourg G; Microbes, Evolution, Phylogeny and Infection, Aix-Marseille Université, UM 63, CNRS 7278, IRD 198, Inserm 1095, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2019 Jan; Vol. 8 (1), pp. e00621. Date of Electronic Publication: 2018 Apr 14.
DOI: 10.1002/mbo3.621
Abstrakt: Intestinimonas massiliensis sp. nov strain GD2 T is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono-genomics, it is strictly anaerobic, nonspore-forming, rod-shaped, with catalase- and oxidase-negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein-coding genes and 62 RNA-coding genes. Strain GD2 T significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.
(© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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