mRNA structure determines specificity of a polyQ-driven phase separation.

Autor: Langdon EM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Qiu Y; Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA., Ghanbari Niaki A; Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA., McLaughlin GA; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Weidmann CA; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Gerbich TM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Smith JA; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Crutchley JM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Termini CM; Division of Hematology/Oncology, Department of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA., Weeks KM; Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Myong S; Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA., Gladfelter AS; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. amyglad@unc.edu.; Marine Biological Laboratory, Woods Hole, MA 02543, USA.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2018 May 25; Vol. 360 (6391), pp. 922-927. Date of Electronic Publication: 2018 Apr 12.
DOI: 10.1126/science.aar7432
Abstrakt: RNA promotes liquid-liquid phase separation (LLPS) to build membraneless compartments in cells. How distinct molecular compositions are established and maintained in these liquid compartments is unknown. Here, we report that secondary structure allows messenger RNAs (mRNAs) to self-associate and determines whether an mRNA is recruited to or excluded from liquid compartments. The polyQ-protein Whi3 induces conformational changes in RNA structure and generates distinct molecular fluctuations depending on the RNA sequence. These data support a model in which structure-based, RNA-RNA interactions promote assembly of distinct droplets and protein-driven, conformational dynamics of the RNA maintain this identity. Thus, the shape of RNA can promote the formation and coexistence of the diverse array of RNA-rich liquid compartments found in a single cell.
(Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze: MEDLINE
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