| Autor: |
Khan AZ; 1Department of Medical Biochemistry,Oslo University Hospital,Kirkeveien 166,P.O. Box 4956,Nydalen,0424 Oslo,Norway., Utheim TP; 1Department of Medical Biochemistry,Oslo University Hospital,Kirkeveien 166,P.O. Box 4956,Nydalen,0424 Oslo,Norway., Reppe S; 1Department of Medical Biochemistry,Oslo University Hospital,Kirkeveien 166,P.O. Box 4956,Nydalen,0424 Oslo,Norway., Sandvik L; 10Department of Periodontology,Institute of Clinical Odontology, Faculty of Dentistry,University of Oslo,Sognsvannsveien 10,P.O. Box 1052,0316 Oslo,Norway., Lyberg T; 1Department of Medical Biochemistry,Oslo University Hospital,Kirkeveien 166,P.O. Box 4956,Nydalen,0424 Oslo,Norway., Roald BB; 2Institute of Clinical Medicine, Faculty of Medicine,University of Oslo,P.O. Box 1171,Blindern,0318 Oslo,Norway., Ibrahim IB; 2Institute of Clinical Medicine, Faculty of Medicine,University of Oslo,P.O. Box 1171,Blindern,0318 Oslo,Norway., Eidet JR; 1Department of Medical Biochemistry,Oslo University Hospital,Kirkeveien 166,P.O. Box 4956,Nydalen,0424 Oslo,Norway. |
| Abstrakt: |
The advancement of human retinal pigment epithelial cell (hRPE) replacement therapy is partly dependent on optimization of cell culture, cell preservation, and storage medium. This study was undertaken to search for a suitable storage temperature and storage medium for hRPE. hRPE monolayer sheets were cultured under standard conditions at 37°C and then randomized for storage at six temperatures (4, 16, 20, 24, 28, and 37°C) for 7 days. After revealing a suitable storage temperature, hRPE sheets were subsequently stored with and without the silk protein sericin added to the storage medium. Live/dead assay, light microscopy, pH, and phenotypic expression of various proteins were used to assess cell cultures stored at different temperatures. After 7 days of storage, hRPE morphology was best preserved at 4°C. Addition of sericin to the storage medium maintained the characteristic morphology of the preserved cells, and improved pigmentation and levels of pigmentation-related proteins in the cultured hRPE sheets following a 7-day storage period at 4°C. |
