Identification of a new variant of Chlamydia trachomatis in Mexico.

Autor: Escobedo-Guerra MR; Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico; Unidad Médica de Alta Especialidad, Hospital de Especialidades, Centro Médico Nacional La Raza, IMSS, CDMX, Mexico., Katoku-Herrera M; Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico., Lopez-Hurtado M; Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico., Villagrana-Zesati JR; Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico., de Haro-Cruz MJ; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, del Instituto Politécnico Nacional, CDMX, Mexico., Guerra-Infante FM; Departamento de Infectología, Instituto Nacional de Perinatología, CDMX, Mexico; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, del Instituto Politécnico Nacional, CDMX, Mexico. Electronic address: fguerra_96@yahoo.com.
Jazyk: English; Spanish; Castilian
Zdroj: Enfermedades infecciosas y microbiologia clinica (English ed.) [Enferm Infecc Microbiol Clin (Engl Ed)] 2019 Feb; Vol. 37 (2), pp. 93-99. Date of Electronic Publication: 2018 Apr 07.
DOI: 10.1016/j.eimc.2018.02.008
Abstrakt: Introduction: Chlamydia trachomatis is one of the main etiological agents of sexually transmitted infections worldwide. In 2006, a Swedish variant of C. trachomatis (Swedish-nvCT), which has a deletion of 377bp in the plasmid, was reported. In Latin America, Swedish-nvCT infections have not been reported. We investigated the presence of Swedish-nvCT in women with infertility in Mexico.
Methods: Swedish-nvCT was searched in 69C. trachomatis positive samples from 2339 endocervical specimens. We designed PCR primers to identify the deletion in the plasmid in the ORF1, and the presence of a repeated 44bp in the ORF3. The sample with the deletion was genotyped with the genes of the major outer membrane protein A (ompA) and the polymorphic membrane protein (pmpH).
Results: The deletion was detected in one of the 69 samples positive C. trachomatis of 2339 endocervical exudates. The nucleotide sequence analysis of the ompA shows a high degree of similarity with the Swedish nvCT (98%), however the variant found belongs to serovar D. The nucleotide sequence of the pmpH gene associates to the variant found in the genitourinary pathotype of the Swedish-nvCT but in different clusters.
Conclusions: Our results revealed the presence of a new variant of C. trachomatis in Mexican patients. This variant found in Mexico belongs to serovar D based on the in silico analysis of the ompA and pmpH genes and differs to the Swedish-nvCT (serovars E). For these variants of C. trachomatis that have been found it is necessary to carry out a more detailed analysis, although the role of this mutation has not been demonstrated in the pathogenesis.
(Copyright © 2018 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.)
Databáze: MEDLINE
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