Effects of lidocaine and the inclusion complex with 2-hydroxypropyl-β-cyclodextrin on cell viability and proliferation of oral squamous cell carcinoma.

Autor: Ferreira LEN; Department Foundation Sciences, School of Dental Medicine, East Carolina University, Greenville, NC, USA., Antunes GBM; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil., Muniz BV; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil., Burga-Sánchez J; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil., de Melo NFS; Department of Immunology and Molecular Biology, São Leopoldo Mandic Research Center, Campinas, SP, Brazil., Groppo FC; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil., Fraceto LF; Laboratory of Environmental Nanotechnology, Institute of Science and Technology of Sorocaba, São Paulo State University (UNESP), Sorocaba, SP, Brazil., Volpato MC; Department of Physiological Sciences, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil.
Jazyk: angličtina
Zdroj: The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2018 Jul; Vol. 70 (7), pp. 874-882. Date of Electronic Publication: 2018 Apr 06.
DOI: 10.1111/jphp.12917
Abstrakt: Objectives: Squamous cell carcinoma (SCC) is a malignant disease that affects the oral cavity. Lidocaine has shown antiproliferative and cytotoxic activity on several cell types. The rapid dispersion is a limitation issue; however, the complexation in cyclodextrin improved pharmacological features and modified the drug release. This study investigated the effects of lidocaine (lido) complexed with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD-lido) on cell viability and proliferation of human tongue squamous cell carcinoma SCC9 and SCC25.
Methods: The complex formation was confirmed by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Cells SCC9 and SCC25 were exposed to lido and HP-β-CD-lido (40-4000 μm), and the effects on cell viability (MTT) and antiproliferative activity (SRB) were tested.
Key Findings: Differential scanning calorimetry and SEM results demonstrated the occurrence of host-guest interaction. Lido and HP-β-CD-lido (4000 μm) significantly reduced the viability of SCC9 cells to 83% and 63%, respectively. The viability of SCC25 treated with lido, and HP-β-CD-lido (4000 μm) was 71% and 44%, respectively. Lido (4000 μm) reduced the proliferation of SCC9 and SCC25 to 39.5% and 23.7%, respectively. HP-β-CD-lido (4000 μm) was cytotoxic for both cell lines.
Conclusions: HP-β-CD was able to potentiate the in vitro cytotoxic effects of lidocaine on human squamous cell carcinoma.
(© 2018 Royal Pharmaceutical Society.)
Databáze: MEDLINE
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