A single-step transconjugation system for gene deletion in Aggregatibacter actinomycetemcomitans.

Autor: Stumpp NS; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Germany., Konze SA; Institute of Clinical Biochemistry, Hannover Medical School, Germany., Gerardy-Schahn R; Institute of Clinical Biochemistry, Hannover Medical School, Germany., Stiesch M; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Germany. Electronic address: stiesch.meike@mh-hannover.de., Buettner FFR; Institute of Clinical Biochemistry, Hannover Medical School, Germany. Electronic address: buettner.falk@mh-hannover.de.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2018 May; Vol. 148, pp. 74-77. Date of Electronic Publication: 2018 Apr 05.
DOI: 10.1016/j.mimet.2018.04.002
Abstrakt: Aggregatibacter (A.) actinomycetemcomitans is a periodontopathogenic bacterium causing aggressive periodontitis. Here we describe a single-step transconjugation system as novel and easily applicable protocol for site-specific genetic manipulation of A. actinomycetemcomitans. Deletion of PgaC, which is involved in the synthesis of biofilm matrix, led to a reduced biofilm formation.
(Copyright © 2018. Published by Elsevier B.V.)
Databáze: MEDLINE
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