| Autor: |
Nilsson LKJ; Department of Ecology, Swedish University of Agricultural Sciences (SLU), 750 07 Uppsala, Sweden.; Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, Sweden., Sharma A; Insect resistance group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.; AAiM Edupoint, Janak Puri, New Delhi-110058, India., Bhatnagar RK; Insect resistance group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India., Bertilsson S; Department of Ecology and Genetics, Limnology and Science for Life Laboratory, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, Sweden., Terenius O; Department of Ecology, Swedish University of Agricultural Sciences (SLU), 750 07 Uppsala, Sweden.; Department of Cell and Molecular Biology, Microbiology, Uppsala University, Box 596, 751 24 Uppsala, Sweden. |
| Abstrakt: |
Water-storage containers are common in households where access to water is scarce and often act as breeding sites for vector mosquitoes. Bacteria in these containers may be important for attracting or repelling ovipositing mosquitoes. We hypothesized that bacterial community composition in water-storage containers would represent either inhibitory or suitable environmental conditions for mosquito larvae. To investigate this, we characterized the bacterial community composition in water-storage containers and correlated these communities to Aedes and Anopheles larval densities. Water samples were collected over two years from 13 containers in an Indian village and analyzed by high throughput 16S rRNA gene amplicon sequencing. Comparisons of bacterial community composition between water with and without mosquito larvae showed that Xanthomonadaceae, Comamonadaceae and Burkholderiaceae were more common (P < 0.05) in absence of larvae, while Lachnospiraceae, Synechococcaceae, Alcaligenaceae and Cryomorphaceae were more common (P < 0.05) in presence of larvae. Indicator analysis identified operational taxonomic units designated as CL500-29 marine group (Acidimicrobiaceae) and FukuN101 (Microbacteriaceae) for absence and presence of larvae, respectively. These results contribute to the understanding of which bacteria, directly or indirectly, can be linked to absence or presence of mosquitoes around households and set the basis for potential measures to be taken against these vector mosquitoes. |
