Passion Fruit Chlorotic Mottle Virus: Molecular Characterization of a New Divergent Geminivirus in Brazil.

Autor: Fontenele RS; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. rfontene@asu.edu.; The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. rfontene@asu.edu., Abreu RA; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. rayannyalexandre@gmail.com.; Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité, PB 58175-000, Brazil. rayannyalexandre@gmail.com., Lamas NS; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. nslamas@gmail.com., Alves-Freitas DMT; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. dionebio@gmail.com., Vidal AH; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. andrezactg@hotmail.com.; Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité, PB 58175-000, Brazil. andrezactg@hotmail.com., Poppiel RR; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. raulpoppiel@gmail.com., Melo FL; Departmento de Biologia Celular, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília, DF 70910-900, Brazil. flucasmelo@gmail.com., Lacorte C; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. cristiano.lacorte@embrapa.br., Martin DP; Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory 7925, South Africa. darrenpatrickmartin@gmail.com., Campos MA; Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité, PB 58175-000, Brazil. magnoliacp@gmail.com., Varsani A; The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. arvind.varsani@asu.edu.; Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory, Cape Town 7701, South Africa. arvind.varsani@asu.edu., Ribeiro SG; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF 70770-017, Brazil. simone.ribeiro@embrapa.br.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2018 Apr 02; Vol. 10 (4). Date of Electronic Publication: 2018 Apr 02.
DOI: 10.3390/v10040169
Abstrakt: Brazil is one of the major passion fruit producers worldwide. Viral diseases are among the most important constraints for passion fruit production. Here we identify and characterize a new passion fruit infecting-virus belonging to the family Geminiviridae : passion fruit chlorotic mottle virus (PCMoV). PCMoV is a divergent geminivirus unlike previously characterized passion fruit-infecting geminiviruses that belonged to the genus Begomovirus . Among the presently known geminiviruses, it is most closely related to, and shares ~62% genome-wide identity with citrus chlorotic dwarf associated virus (CCDaV) and camelia chlorotic dwarf associated virus (CaCDaV). The 3743 nt PCMoV genome encodes a capsid protein (CP) and replication-associated protein (Rep) that respectively share 56 and 60% amino acid identity with those encoded by CaCDaV. The CPs of PCMoV, CCDaV, and CaCDaV cluster with those of begomovirus whereas their Reps with those of becurtoviruses. Hence, these viruses likely represent a lineage of recombinant begomo-like and becurto-like ancestral viruses. Furthermore, PCMoV, CCDaV, and CaCDaV genomes are ~12-30% larger than monopartite geminiviruses and this is primarily due to the encoded movement protein (MP; 891-921 nt) and this MP is most closely related to that encoded by the DNA-B component of bipartite begomoviruses. Hence, PCMoV, CCDaV, and CaCDaV lineage of viruses may represent molecules in an intermediary step in the evolution of bipartite begomoviruses (~5.3 kb) from monopartite geminiviruses (~2.7-3 kb). An infectious clone of PCMoV systemically infected Nicotiana benthamina , Arabidopsis thaliana , and Passiflora edulis .
Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
Databáze: MEDLINE
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