Extended exposure duration of cultured intestinal epithelial cell monolayers in characterizing hazardous and non-hazardous proteins.

Autor: Zimmermann C; DuPont Pioneer, Johnston, IA 50131, United States., Eaton AD; Department of Pediatrics, Mucosal Immunology & Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, United States., Lanter BB; Department of Pediatrics, Mucosal Immunology & Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, United States., Roper J; DuPont Haskell, 1090 Elkton Road, Newark, DE 19714, United States., Hurley BP; Department of Pediatrics, Mucosal Immunology & Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, United States. Electronic address: bphurley@partners.org., Delaney B; DuPont Pioneer, Johnston, IA 50131, United States.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2018 May; Vol. 115, pp. 451-459. Date of Electronic Publication: 2018 Mar 31.
DOI: 10.1016/j.fct.2018.03.047
Abstrakt: Recent studies suggest that human derived intestinal epithelial cells (IECs) cultured as polarized monolayers on Transwell ® filters may respond differently when exposed to hazardous and non-hazardous proteins. This experimental platform was based on apical exposure of IEC monolayers to test proteins for 24 h followed by assessment of barrier integrity and cell viability. In this study, Caco-2 and T84 IEC polarized monolayers were evaluated for barrier integrity and cytotoxicity following exposure to hazardous and non-hazardous proteins for 24, 48 and 72 h. Hazardous proteins included Clostridium difficile toxin A (ToxA), Streptolysin O (SLO), Wheat Germ Agglutinin (WGA), and Phaseolus vulgaris haemagglutinin-E (PHA-E). Non-hazardous proteins included bovine serum albumin (BSA), porcine serum albumin (PSA), and fibronectin (Fbn). In general, evidence of diminished barrier integrity or cell viability observed following exposure to hazardous proteins for 24 h was more pronounced after 48 and 72 h for both IEC monolayers. Non-hazardous proteins exhibiting no impact following 24 h of exposure elicited minimal effects over longer exposure durations. These results support the utility of using cultured human IEC polarized monolayers to differentiate between hazardous and non-hazardous proteins and suggest that longer durations of exposure may further improve the ability to distinguish between them.
(Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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