| Autor: |
Ramakrishnan MA; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Pundkar CY; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Fayaz A; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., ChandraSekar S; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Mageswary R; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Ashokkumar D; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Bano R; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Muthuchelvan D; 1Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138 India., Nandi S; 2Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India., Gupta VK; 2Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India. |
| Abstrakt: |
Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis is one of the high economic importance diseases of cattle and caused by bovine herpesvirus1 (BoHV1). Based on the restriction endonuclease fingerprinting of viral DNA, the BoHV1 can be divided into three subtypes viz., BoHV1.1, 1.2a, and 1.2b. Since this method requires a pure viral DNA, it is time-consuming and labour intense. In the current study, the UL0.5 gene based PCR sequencing has been used for the subtyping of BoHV1. Out of five isolates, four had BoHV1-like signatures and one isolate had BoHV1.2-like signatures. Further, these viruses phylogenetically clustered under the respective subtypes. These results indicate that the UL 0.5 gene based PCR sequencing could be used as an alternate method of subtyping of BoHV1. |
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