A porous graphitized carbon LC-ESI/MS method for the quantitation of metronidazole and fluconazole in breast milk and human plasma.

Autor: Geballa-Koukoula A; National and Kapodistrian University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Laboratory of Pharmaceutical Analysis, Panepistimiopolis, Zografou 157 71, Athens, Greece., Panderi I; National and Kapodistrian University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Laboratory of Pharmaceutical Analysis, Panepistimiopolis, Zografou 157 71, Athens, Greece. Electronic address: irenepanderi@gmail.com., Zervas K; National and Kapodistrian University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Laboratory of Pharmaceutical Analysis, Panepistimiopolis, Zografou 157 71, Athens, Greece., Geballa-Koukoulas K; National and Kapodistrian University of Athens, Department of Biology, Division of Zoology-Marine Biology, Panepistimiopolis, Zografou 157 71, Athens, Greece., Kavvalou E; University of Crete, School of Medicine, Department of Dermatology, Heraklion 71110, Crete, Greece., Panteri-Petratou E; Sismanoglio General Hospital, Sismanogliou 39, 151 27 Melissia, Greece., Vourna P; General- Maternity District Hospital Elena Venizelou, Milk Bank and Maternal Breast-feeding Department, 2 Helena Venizelou Square, 115 21, Athens, Greece., Gennimata D; General Hospital 'Korgialenio-Benakio National Red Cross', Erithrou Stavrou 1, 115 26, Athens, Greece.
Jazyk: angličtina
Zdroj: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2018 May 01; Vol. 1084, pp. 175-184. Date of Electronic Publication: 2018 Mar 22.
DOI: 10.1016/j.jchromb.2018.03.034
Abstrakt: Information on drug transfer into the breast milk is essential to protect the infant from undesirable adverse effects of maternal consumption of drugs and to allow effective pharmacological treatment of breastfeeding mothers. Metronidazole and fluconazole are two drugs frequently used in nursing women to treat various infections, thus questioning infant's safety due to drug exposure through breast milk. In this article a porous graphitized carbon LC/ESI-MS assay was developed for the quantitation of metronidazole and fluconazole in breast milk and human plasma. The assay was based on the use of 150 μL of biological samples, following acetonitrile precipitation of proteins and filtration that enabled injection into the LC/ESI-MS system. All analytes and the internal standard, ropinirole, were separated by using a porous graphitized carbon analytical column (150 × 2.1 mm i.d., particle size 5 μm) with isocratic elution. The mobile phase consists of 55% acetonitrile in water acidified with 0.1% concentrated formic acid and pumped at a flow rate of 0.25 mL min -1 . The assay was linear over a concentration range of 0.1 to 15 μg mL -1 for all analytes in both biological samples. Intermediate precision was found to be <8.4% over the tested concentration ranges. A run time of <5 min for each sample made it possible to analyze a large number of biological samples per day. The method is the first reported application for the analysis of metronidazole and fluconazole in both breast milk and human plasma and it can be used to support a wide range of clinical studies.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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