Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting.

Autor: Mackie M; Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.; Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Rüther P; Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Samodova D; Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Di Gianvincenzo F; Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark., Granzotto C; Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark., Lyon D; Disease Systems Biology Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Peggie DA; Scientific Department, National Gallery London, Trafalgar Square, London, WC2N 5DN, UK., Howard H; Scientific Department, National Gallery London, Trafalgar Square, London, WC2N 5DN, UK., Harrison L; Conservation Department, National Gallery London, Trafalgar Square, London, WC2N 5DN, UK., Jensen LJ; Disease Systems Biology Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Olsen JV; Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Blegdamsvej 3b, 2200, Copenhagen, Denmark., Cappellini E; Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2018 Jun 18; Vol. 57 (25), pp. 7369-7374. Date of Electronic Publication: 2018 Apr 26.
DOI: 10.1002/anie.201713020
Abstrakt: Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.
(© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
Databáze: MEDLINE
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