Establishment of an in vivo turkey model for the study of flexor tendon repair.

Autor: Kadar A; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota.; Orthopedic Division, Tel Aviv University, Tel Aviv, Israel., Liu H; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota., Vrieze AM; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota., Meier TR; Department of Comparative Medicine, Mayo Clinic, Rochester, Minnesota., Thoreson AR; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota., Amadio PC; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota., Moran SL; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota., Zhao C; Department of Orthopedic Surgery, Orthopedic Biomechanics Laboratory, Mayo Clinic, Rochester, Minnesota.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2018 Sep; Vol. 36 (9), pp. 2497-2505. Date of Electronic Publication: 2018 Apr 25.
DOI: 10.1002/jor.23904
Abstrakt: Flexor tendon injuries are common and pose a clinical challenge for functional restoration. The purpose of our study was to assess the adequacy of the turkey as a large animal model for flexor tendon injuries in vivo. Twenty-four male turkeys underwent surgical flexor tendon cut and repair. Turkeys were allocated to five groups postoperatively: (1) foot casted in extension and sacrificed after 3 weeks; (2) foot casted in extension and sacrificed after 6 weeks; (3) foot casted in flexion and sacrificed after 3 weeks; (4) foot casted in flexion and sacrificed after 6 weeks; and (5) foot casted in flexion for 6 weeks and then free roaming allowed for an additional 3 weeks before sacrifice. After sacrifice, digits were collected and analyzed for adhesion formation, healing at the macrolevel and histologically, and biomechanical properties-including friction, work of flexion, stiffness, and strength of repair. All turkeys survived anesthesia and surgery. Tendon rupture occurred in all extension casts and in 11% of those casted in flexion. Friction and work of flexion were significantly higher in the repaired digit than the control digit. There was a correlation between duration of immobilization and repair strength. Histologically, the tendon healed with tenocytes migrating into the gap and producing collagen fibers. We have, for the first time, studied flexor tendon injury and repair using turkeys in terms of anesthesia, surgical procedures, postoperative care, and animal husbandry. The findings regarding functional and histological results from this novel avian model were comparable to the most commonly used mammal model. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2497-2505, 2018.
(© 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)
Databáze: MEDLINE
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