LC-MS analysis and cytoprotective effect against the mercurium and aluminium toxicity by bioactive products of Psidium brownianum Mart. ex DC.

Autor: Sobral-Souza CE; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato CE, Brazil; Faculdade Vale do Salgado, Icó CE, Brazil., Silva ARP; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato CE, Brazil., Leite NF; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato CE, Brazil., Costa JGM; Laboratory of Natural Products Research, Regional University of Cariri, Crato CE, Brazil., Menezes IRA; Laboratory of Pharmacology and Molecular Chemistry, Regional University of Cariri, Crato CE, Brazil., Cunha FAB; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato CE, Brazil., Rolim LA; Center of Drug, Remedies and Food Analysis, Federal University of the São Francisco Valley, Petrolina PE, Brazil., Coutinho HDM; Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato CE, Brazil. Electronic address: hdmcoutinho@gmail.com.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2019 May 15; Vol. 370, pp. 54-62. Date of Electronic Publication: 2018 Mar 21.
DOI: 10.1016/j.jhazmat.2018.03.039
Abstrakt: This study aimed to verify the chelating, antioxidant and cytoprotective activities of Psidium brownianum Mart. Ex DC against mercury and aluminum. The ethanolic extract, as well as the tannic and flavonoid fractions, were prepared and subjected to liquid chromatography-mass spectrometry analysis. Ferric ion reduction and antioxidant activity measurement using the FRAP method were performed with P. brownianum. After determining the sub-allelopathic doses, germination tests using Lactuca sativa (lettuce) seeds were performed. The main compounds identified in the extract and fractions were: quercetin and its derivatives; myricetin and its derivatives; gallic acid; ellagic acid; quinic acid and gallocatechin. The Minimum Inhibitory Concentration (MIC) for all samples were ≥ 1024 μg/mL. The flavonoid fraction in association with mercury chloride demonstrated cytoprotection (p < 0.001). The sub-allelopathic concentration used was 64 μg/mL. The extract and fractions were cytoprotective for radicles and caulicles when assayed in association with mercury and against aluminum for radicles. This suggests that the P. brownianum extract and its fractions present cytoprotective activity, possibly related to the antioxidant effect of secondary metabolites, especially flavonoids.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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