Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi.

Autor: Killion HJ; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox)., Edwards W; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox)., Jennings-Gaines J; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox)., Wood M; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox)., Fox K; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox)., Sondgeroth K; Wildlife Health Laboratory (Killion, Edwards, Jennings-Gaines, Wood), Veterinary Services, Wyoming Game and Fish Department, Laramie, WY.; Department of Veterinary Sciences, University of Wyoming, Laramie, WY (Sondgeroth).; Wildlife Health Program, Colorado Division of Parks and Wildlife, Fort Collins, CO (Fox).
Jazyk: angličtina
Zdroj: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2018 Jul; Vol. 30 (4), pp. 589-592. Date of Electronic Publication: 2018 Mar 30.
DOI: 10.1177/1040638717753497
Abstrakt: A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukotoxin gene of B. trehalosi and provides a rapid and direct approach for detecting leukotoxin-producing forms of this organism from samples containing mixed species of leukotoxin-positive Pasteurellaceae.
Databáze: MEDLINE