No significant regulation of bicoid mRNA by Pumilio or Nanos in the early Drosophila embryo.

Autor: Wharton TH; Departments of Molecular Genetics & Cancer Biology and Genetics, Center for RNA Biology Ohio State University Columbus, OH., Nomie KJ; Department of Lymphoma/Myeloma MD Anderson Cancer Center Holcombe Blvd. Houston, TX., Wharton RP; Departments of Molecular Genetics & Cancer Biology and Genetics, Center for RNA Biology Ohio State University Columbus, OH.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Mar 30; Vol. 13 (3), pp. e0194865. Date of Electronic Publication: 2018 Mar 30 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0194865
Abstrakt: Drosophila Pumilio (Pum) is a founding member of the conserved Puf domain class of RNA-binding translational regulators. Pum binds with high specificity, contacting eight nucleotides, one with each of the repeats in its RNA-binding domain. In general, Pum is thought to block translation in collaboration with Nanos (Nos), which exhibits no binding specificity in isolation but is recruited jointly to regulatory sequences containing a Pum binding site in the 3'-UTRs of target mRNAs. Unlike Pum, which is ubiquitous in the early embryo, Nos is tightly restricted to the posterior, ensuring that repression of its best-characterized target, maternal hunchback (hb) mRNA, takes place exclusively in the posterior. An exceptional case of Nos-independent regulation by Pum has been described-repression of maternal bicoid (bcd) mRNA at the anterior pole of the early embryo, dependent on both Pum and conserved Pum binding sites in the 3'-UTR of the mRNA. We have re-investigated regulation of bcd in the early embryo; our experiments reveal no evidence of a role for Pum or its conserved binding sites in regulation of the perdurance of bcd mRNA or protein. Instead, we find that Pum and Nos control the accumulation of bcd mRNA in testes.
Databáze: MEDLINE
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