Bacterial and Pneumocystis Infections in the Lungs of Gene-Knockout Rabbits with Severe Combined Immunodeficiency.

Autor: Song J; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States., Wang G; Louisiana State University Health Sciences Center, New Orleans, LA, United States., Hoenerhoff MJ; In Vivo Animal Core, University of Michigan Medical School, Ann Arbor, MI, United States., Ruan J; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States., Yang D; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States., Zhang J; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States., Yang J; Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, United States., Lester PA; Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, United States., Sigler R; In Vivo Animal Core, University of Michigan Medical School, Ann Arbor, MI, United States., Bradley M; Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, United States., Eckley S; Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, United States., Cornelius K; Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI, United States., Chen K; Department of Medicine, University of Pittsburgh, Pittsburgh, PA, United States., Kolls JK; Department of Medicine, University of Pittsburgh, Pittsburgh, PA, United States., Peng L; Critical Care Medicine Department, National Institutes of Health, Bethesda, MD, United States., Ma L; Critical Care Medicine Department, National Institutes of Health, Bethesda, MD, United States., Chen YE; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States., Sun F; Wayne State University School of Medicine, Detroit, MI, United States., Xu J; Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, University of Michigan Medical School, Ann Arbor, MI, United States.
Jazyk: angličtina
Zdroj: Frontiers in immunology [Front Immunol] 2018 Mar 09; Vol. 9, pp. 429. Date of Electronic Publication: 2018 Mar 09 (Print Publication: 2018).
DOI: 10.3389/fimmu.2018.00429
Abstrakt: Using the CRISPR/Cas9 gene-editing technology, we recently produced a number of rabbits with mutations in immune function genes, including FOXN1, PRKDC, RAG1, RAG2, and IL2RG. Seven founder knockout rabbits (F0) and three male IL2RG null (-/y) F1 animals demonstrated severe combined immunodeficiency (SCID), characterized by absence or pronounced hypoplasia of the thymus and splenic white pulp, and absence of immature and mature T and B-lymphocytes in peripheral blood. Complete blood count analysis showed severe leukopenia and lymphocytopenia accompanied by severe neutrophilia. Without prophylactic antibiotics, the SCID rabbits universally succumbed to lung infections following weaning. Pathology examination revealed severe heterophilic bronchopneumonia caused by Bordetella bronchiseptica in several animals, but a consistent feature of lung lesions in all animals was a severe interstitial pneumonia caused by Pneumocystis oryctolagi , as confirmed by histological examination and PCR analysis of Pneumocystis genes. The results of this study suggest that these SCID rabbits could serve as a useful model for human SCID to investigate the disease pathogenesis and the development of gene and drug therapies.
Databáze: MEDLINE