HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression.

Autor: Gomes RG; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Brito CAA; Department of Internal Medicine, School of Medicine, Federal University of Pernambuco, Recife, Brazil., Martinelli VF; Department of Internal Medicine, School of Medicine, Federal University of Pernambuco, Recife, Brazil., Santos RND; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Gomes FODS; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Peixoto CA; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Crispim JO; Department of Pharmacology, Federal University of Rio Grande do Norte, Natal, Brazil., Diniz GTN; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil., Donadi EA; Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil., Lucena-Silva N; Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil. Electronic address: norma.lucena@hotmail.com.
Jazyk: angličtina
Zdroj: Human immunology [Hum Immunol] 2018 Jun; Vol. 79 (6), pp. 477-484. Date of Electronic Publication: 2018 Mar 26.
DOI: 10.1016/j.humimm.2018.03.006
Abstrakt: HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder.
(Copyright © 2018 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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