| Autor: |
Ross GMS; RIKILT, Wageningen University and Research, P.O Box 230, 6700 AE, Wageningen, The Netherlands. georgina.ross@wur.nl., Bremer MGEG; RIKILT, Wageningen University and Research, P.O Box 230, 6700 AE, Wageningen, The Netherlands., Nielen MWF; RIKILT, Wageningen University and Research, P.O Box 230, 6700 AE, Wageningen, The Netherlands.; Laboratory of Organic Chemistry, Wageningen University, Helix Building 124, Stippeng 4, 6708 WE, Wageningen, The Netherlands. |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2018 Sep; Vol. 410 (22), pp. 5353-5371. Date of Electronic Publication: 2018 Mar 26. |
| DOI: |
10.1007/s00216-018-0989-7 |
| Abstrakt: |
In this critical review, we provide a comprehensive overview of immunochemical food allergen assays and detectors in the context of their user-friendliness, through their connection to smartphones. Smartphone-based analysis is centered around citizen science, putting analysis into the hands of the consumer. Food allergies represent a significant worldwide health concern and consumers should be able to analyze their foods, whenever and wherever they are, for allergen presence. Owing to the need for a scientific background, traditional laboratory-based detection methods are generally unsuitable for the consumer. Therefore, it is important to develop simple, safe, and rapid assays that can be linked with smartphones as detectors to improve user accessibility. Smartphones make excellent detection systems because of their cameras, embedded flash functions, portability, connectivity, and affordability. Therefore, this review has summarized traditional laboratory-based methods for food allergen detection such as enzyme-linked-immunosorbent assay, flow cytometry, and surface plasmon resonance, and the potential to modernize these methods by interfacing them with a smartphone readout system, based on the aforementioned smartphone characteristics. This is the first review focusing on smartphone-based food-allergen detection methods designed with the intention of being consumer-friendly. Graphical abstract A smartphone-based food allergen detection system in three easy steps (1) sample preparation, (2) allergen detection on a smartphone using antibodies, which then transmits the data wirelessly, (3) analytical results sent straight to smartphone. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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