A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods.

Autor: Hartnell RE; European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth DT4 8UB, United Kingdom., Stockley L; European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth DT4 8UB, United Kingdom., Keay W; European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth DT4 8UB, United Kingdom., Rosec JP; Service Commun des Laboratoires Ministère de l' Economie et des Finances, Montpellier, France., Hervio-Heath D; LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France., Van den Berg H; National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven, The Netherlands., Leoni F; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy., Ottaviani D; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy., Henigman U; Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI - 1000, Ljubljana, Slovenia., Denayer S; Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium., Serbruyns B; Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium., Georgsson F; Icelandic Food and Biotech R&D Vínlandsleið 12, 113 Reykjavík, Iceland., Krumova-Valcheva G; Food Safety Laboratory, 15 Pencho Slaveikov blvd, Sofia 1606, Bulgaria., Gyurova E; Food Safety Laboratory, 15 Pencho Slaveikov blvd, Sofia 1606, Bulgaria., Blanco C; Agencia Española de Sguridad Alimentaria, Centro Nacional de Alimentación, Servicio de Microbiología Alimentaria Carretera de Majadahonda a Pozuelo, Km, 5,2 Majadahonda, 28220 Madrid, Spain., Copin S; ANSES, Laboratoire des produits de la pêche de Boulogne-sur-mer, Boulevard du Bassin Napoléon, 62200 Boulogne sur Mer, France., Strauch E; Bundesinstitut für Risikobewertung (BfR) German Federal Institute for Risk Assessment, Diedersdorfer Weg, 112277 Berlin, Germany., Wieczorek K; Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin, Partyzantow 57, 24-100 Pulawy, Poland., Lopatek M; Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin, Partyzantow 57, 24-100 Pulawy, Poland., Britova A; State Veterinary and Food Institute, Janoskova 1611/58, 026 01 Dolny Kubin, Slovakia., Hardouin G; Association Française de Normalisation, Saint-Denis, France., Lombard B; Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail, Maisons-Alfort, France., In't Veld P; Netherlands Food and Consumer Product Safety Authority, Utrecht, The Netherlands., Leclercq A; Institut Pasteur, Paris, France., Baker-Austin C; European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth DT4 8UB, United Kingdom. Electronic address: craig.baker-austin@cefas.co.uk.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2019 Jan 02; Vol. 288, pp. 58-65. Date of Electronic Publication: 2018 Feb 10.
DOI: 10.1016/j.ijfoodmicro.2018.02.008
Abstrakt: Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872-1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872-2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp.. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.
(Copyright © 2018. Published by Elsevier B.V.)
Databáze: MEDLINE
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