Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis REV1 vaccine produced in bioreactor.

Autor: Curina G; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: g.curina@izsum.it., Nardini R; Istituto Zooprofilattico Sperimentale del Lazio e della Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178, Rome, Italy. Electronic address: roberto.nardini@izslt.it., Corneli S; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy; Department of Experimental Medicine, University of Perugia, Piazzale Gambuli 1, 06132, Perugia, Italy. Electronic address: s.corneli@izsum.it., D'Avino N; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: n.davino@izsum.it., Tentellini M; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: m.tentellini@izsum.it., Montagnoli C; Freelancer, Perugia, Italy. Electronic address: c_montagnoli1@virgilio.it., Severi G; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: g.severi@izsum.it., Forti K; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: k.forti@izsum.it., Paternesi B; Freelancer, Perugia, Italy. Electronic address: beatrice.paternesi@libero.it., Coletti M; Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy. Electronic address: mauro.coletti@unipg.it., Cagiola M; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Gaetano Salvemini 1, 06126, Perugia, Italy. Electronic address: m.cagiola@izsum.it.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2018 Apr; Vol. 198, pp. 44-53. Date of Electronic Publication: 2018 Feb 24.
DOI: 10.1016/j.vetimm.2018.02.010
Abstrakt: The Brucella melitensis REV1 vaccine is the most widely employed vaccine for prophylaxis against brucellosis in sheep and goats. The objective of vaccination is disease control in herds or preventing infection in farms. In this study, we produced REV1 vaccine with a protocol, based on the use of liquid medium in a bioreactor, that resulted efficient, safe, relatively fast, and cost-effective. The live attenuated vaccine produced was tested in mice and sheep to investigate its immunogenicity and efficacy. Seventy-two female BALB/c mice were obtained and subdivided in 2 groups, one was stimulated with 1 × 10 6 colony-forming units (CFUs) of B. melitensis while the other with physiological solution alone and acting as control group. Furthermore, 25 sheep were subdivided into 5 groups: four were inoculated with a B. melitensis dose, ranging from 0.6 × 10 9 and 3.2 × 10 9  CFUs and the other was the control group. In addition, a serological diagnosis was performed for sheep by rapid serum agglutination and the complement-fixation test. Immunocompetent cells from both experiment were collected at different times post vaccination and immunostained to evaluate innate and adaptive-immune responses. In mice flow cytometry was used to detect macrophages, T lymphocytes, dendritic cells, memory cells, naïve cells, natural killer cells, major histocompatibility complex type II, B lymphocytes, regulatory T lymphocytes, T helper lymphocytes, cytotoxic T lymphocytes and recently activated CD4 + and CD8 + lymphocytes. In sheep, macrophages, T helper cells, cytotoxic T lymphocytes, regulatory T lymphocytes, dendritic cells, memory cells and naïve lymphocytes, by the same method, were analyzed. The results showed, both in mice and sheep, that the live, attenuated REV1 vaccine stimulated all immunocompetent cells tested, with a balanced innate and adaptive response. In the sheep experiment, the administered vaccine dose was very important because, at the lower doses, immunological tolerance tended to disappear, while, at the highest dose, the immunological tolerance remained active for a long period. In our experimental conditions, the optimal vaccine dose for sheep was 3.2 × 10 9  CFUs, although a good immune response was found using a dose of 1.6 × 10 9  CFUs. The vaccine produced in this study could be extensively employed in developing countries to control the brucellosis in sheep and goats.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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