An Arabidopsis Clathrin Assembly Protein with a Predicted Role in Plant Defense Can Function as an Adenylate Cyclase.

Autor: Chatukuta P; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. patience.chatukuta@gmail.com., Dikobe TB; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. Tshegofatso.Dikobe@nwu.ac.za., Kawadza DT; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. Dave.Kawadza@nwu.ac.za., Sehlabane KS; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. katlego.sehlabane@gmail.com., Takundwa MM; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. mutsamt@yahoo.com., Wong A; College of Natural, Applied and Health Sciences, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, Zhejiang Province, China. alwong@kean.edu., Gehring C; Department of Chemistry, Biology & Biotechnology, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy. christophandreas.gehring@unipg.it., Ruzvidzo O; Department of Botany, School of Biological Sciences, North-West University, Private Bag X2046, Mmabatho 2735, South Africa. Oziniel.Ruzvidzo@nwu.ac.za.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2018 Mar 23; Vol. 8 (2). Date of Electronic Publication: 2018 Mar 23.
DOI: 10.3390/biom8020015
Abstrakt: Adenylate cyclases (ACs), much like guanylate cyclases (GCs), are increasingly recognized as essential parts of many plant processes including biotic and abiotic stress responses. In order to identify novel ACs, we have applied a search motif derived from experimentally tested GCs and identified a number of Arabidopsis thaliana candidates including a clathrin assembly protein (AT1G68110; AtClAP). AtClAP contains a catalytic centre that can complement the AC-deficient mutant cya A in E. coli , and a recombinant AtClAP fragment (AtClAP 261-379 ) can produce cyclic adenosine 3',5' monophosphate (cAMP) from adenosine triphosphate (ATP) in vitro. Furthermore, an integrated analysis of gene expression and expression correlation implicate cAMP in pathogen defense and in actin cytoskeletal remodeling during endocytic internalization.
Competing Interests: The authors declare no competing interests with regard to contents of this article. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
Databáze: MEDLINE
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