Gene expression profiling reveals a close relationship between follicular lymphoma grade 3A and 3B, but distinct profiles of follicular lymphoma grade 1 and 2.

Autor:	Horn H; Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology, Stuttgart and University of Tübingen, Germany., Kohler C; Statistical Bioinformatics Department, Institute of Functional Genomics, University of Regensburg, Germany., Witzig R; Department of Clinical Pathology, Robert Bosch Krankenhaus, Stuttgart, Germany., Kreuz M; Institute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Germany., Leich E; Institute of Pathology, University of Würzburg, and Comprehensive Cancer Center Mainfranken, Würzburg, Germany., Klapper W; Department of Pathology, Hematopathology Section, University Hospital Schleswig-Holstein Campus Kiel/Christian-Albrechts University Kiel, Germany., Hummel M; Institute of Pathology, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Germany., Loeffler M; Institute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Germany., Trümper L; Department of Hematology and Oncology, Georg-August University of Göttingen, Germany., Spang R; Statistical Bioinformatics Department, Institute of Functional Genomics, University of Regensburg, Germany., Rosenwald A; Institute of Pathology, University of Würzburg, and Comprehensive Cancer Center Mainfranken, Würzburg, Germany., Ott G; Department of Clinical Pathology, Robert Bosch Krankenhaus, Stuttgart, Germany german.ott@rbk.de.
Jazyk:	angličtina
Zdroj:	Haematologica [Haematologica] 2018 Jul; Vol. 103 (7), pp. 1182-1190. Date of Electronic Publication: 2018 Mar 22.
DOI:	10.3324/haematol.2017.181024
Abstrakt:	A linear progression model of follicular lymphomas (FL) FL1, FL2 and FL3A has been favored, since FL3A often co-exist with an FL1/2 component. FL3B, in contrast, is thought to be more closely related to diffuse large B-cell lymphoma (DLBCL), and both are often simultaneously present in one tumor (DLBCL/FL3B). To obtain more detailed insights into follicular lymphoma progression, a comprehensive analysis of a well-defined set of FL1/2 (n=22), FL3A (n=16), FL3B (n=6), DLBCL/FL3B (n=9), and germinal center B-cell-type diffuse large B-cell lymphoma (n=45) was undertaken using gene expression profiling, immunohistochemical stainings and genetic analyses by fluorescence in situ hybridization. While immunohistochemical (CD10, IRF4/MUM1, Ki67, BCL2, BCL6) and genetic profiles (translocations of BCL2, BCL6 and MYC) delineate FL1-3A from FL3B and DLBCL/FL3B, significant differences were observed between FL1/2 and FL3A upon gene expression profiling. Interestingly, FL3B turned out to be closely related to FL3A, not categorizing within a separate gene expression cluster, and both FL3A and FL3B showed overlapping profiles in between FL1/2 and diffuse large B-cell lymphoma. Finally, based upon their gene expression pattern, DLBCL/FL3B represent a composite form of FL3B and DLBCL, with the majority of samples more closely resembling the latter. The fact that gene expression profiling clearly separated FL1/2 from both FL3A and FL3B suggests a closer biological relationship between the latter. This notion, however, is in contrast to immunohistochemical and genetic profiles of the different histological FL subtypes that point to a closer relationship between FL1/2 and FL3A, and separates them from FL3B. (Copyright© 2018 Ferrata Storti Foundation.)
Databáze:	MEDLINE
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