Effect of short- and medium-term toxicity of doxorubicin on spermatogenesis in adult Wistar rats.

Autor: Silva RC; Laboratory of Cellular Biology, Department of Basic Sciences, Federal University of Jequitinhonha and Mucuri Valleys,Alto da Jacuba, Diamantina, Minas Gerais, Brazil. Electronic address: robson.silva@ufvjm.edu.br., Britto DMC; Laboratory of Cellular Biology, Department of Basic Sciences, Federal University of Jequitinhonha and Mucuri Valleys,Alto da Jacuba, Diamantina, Minas Gerais, Brazil., de Fátima Pereira W; Immunology Laboratory, Integrated Health Research Center, Federal University of Jequitinhonha and Mucuri Valleys, Alto da Jacuba, Diamantina, Minas Gerais, Brazil., Brito-Melo GEA; Immunology Laboratory, Integrated Health Research Center, Federal University of Jequitinhonha and Mucuri Valleys, Alto da Jacuba, Diamantina, Minas Gerais, Brazil., Machado CT; Laboratory of Cellular Biology, Department of Basic Sciences, Federal University of Jequitinhonha and Mucuri Valleys,Alto da Jacuba, Diamantina, Minas Gerais, Brazil., Pedreira MM; Department of Animal Sciences, Federal University of Jequitinhonha and Mucuri Valleys, Alto da Jacuba, Diamantina, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2018 Jun; Vol. 18 (2), pp. 169-176. Date of Electronic Publication: 2018 Mar 20.
DOI: 10.1016/j.repbio.2018.03.002
Abstrakt: Doxorubicin (DXR) is a widely used chemotherapeutic anticancer agent that has potent activity against several solid and non-solid human malignant tumors, including childhood malignancies. However, DXR has serious toxic effects on tissues with rapid cell cycles, such as myeloid and lymphatic tissues, intestinal mucosa, testes and ovaries. In the present study, the short- and medium-term toxic effects of DXR on the reproductive system of male Wistar rats were evaluated using morphometric and stereological tools to quantify damage to the seminiferous epithelium. Adult male Wistar rats were treated with dose of 7.5 mg/kg of DXR and were sacrificed at seven, 14, 21 and 28 days after treatment. The testes were fixed in glutaraldehyde solution, routinely processed and embedded in plastic for evaluation under a light microscope. A significant reduction in testis weight was found as a result of massive germ cell apoptosis. Differences in comparison to the control group were found in the relative frequency of all stages of the seminiferous epithelium cycle, with significant differences for stages VIII-XI. Apoptosis significantly decreased the number of pachytene spermatocytes in the stages evaluated (I, II-III and VIII) at seven and 14 days. At 21 and 28 days after treatment, the testes exhibited the massive loss of germ cells that resulted in a missing cell layer. Moreover, reductions in the height of seminiferous tubules, tubular diameter and tubular compartment as well as an increase in the intertubular compartment were found in the period studied.
(Copyright © 2018 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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