Quantitative measurement of oxidative damage in erythrocytes as indicator in benzene intoxications.

Autor: Martínez-Rodríguez JL; a Laboratorio de microRNAs de la Unidad Académica de Ciencias Biológicas de la Universidad Autónoma de Zacatecas , Zacatecas , Mexico.; b Escuela de Medicina de la Universidad Autónoma de Durango campus Zacatecas , Zacatecas , Mexico., Gutiérrez-Hernández R; c Programa de Nutrición de la Unidad Académica de Enfermería de la Universidad Autónoma de Zacatecas , Mexico , Mexico., Reyes-Estrada CA; c Programa de Nutrición de la Unidad Académica de Enfermería de la Universidad Autónoma de Zacatecas , Mexico , Mexico.; d Maestría en Ciencias de la Salud de la Unidad Académica de Medicina Humana y C.S. de la Universidad Autónoma de Zacatecas , Zacatecas , Mexico., Granados-López AJ; a Laboratorio de microRNAs de la Unidad Académica de Ciencias Biológicas de la Universidad Autónoma de Zacatecas , Zacatecas , Mexico., Arcos-Ortega T; b Escuela de Medicina de la Universidad Autónoma de Durango campus Zacatecas , Zacatecas , Mexico., López JA; a Laboratorio de microRNAs de la Unidad Académica de Ciencias Biológicas de la Universidad Autónoma de Zacatecas , Zacatecas , Mexico.; e Doctorado en Ciencias Básicas , Universidad Autónoma de Zacatecas , Zacatecas , Mexico.
Jazyk: angličtina
Zdroj: Toxicology mechanisms and methods [Toxicol Mech Methods] 2018 Jul; Vol. 28 (6), pp. 450-460. Date of Electronic Publication: 2018 Apr 12.
DOI: 10.1080/15376516.2018.1455786
Abstrakt: The metabolism of aromatic hydrocarbons by the organism forms products that cause cell death depending on the type of exposure. Benzene exposure has been linked to oxidative stress, hepatic damage, aplastic anemia, and hematopoietic cancer as lymphoid and myeloid leukemia. However, there are not fast methods to evaluate chronic benzene exposure in human blood. The objective of this work was the evaluation of the correlation between oxidative damage with benzene exposure and the level of cellular plasma membrane stability (CPMS) in erythrocytes to use it as a future indicator to determine the grade of benzene intoxications. CPMS in vitro assays were used to evaluate damage for benzene, toluene, and xylene. Erythrocytes CPMS assays in vitro shows a progressive reduction with benzene, toluene, and xylene suggesting that aromatic hydrocarbons complexity favors CPMS damage. Eight groups of Wistar rats (n = 5) were used to study the level of damage on CPMS by acute and chronic benzene administration. Enzymatic, metabolic, histological, and oxidative damage tests were performed. Acute administration (100 μL/100 g/single dose) showed a decrease of 66.7% in CPMS, while 63.6% for chronic administration (5 μL/100 g/every 2 days/3 months) showing a correlation with liver damage principally (transaminases activity increase, glycogen level decrease, and high oxidative damage). Tissue damage was observed in bone marrow, kidney, spleen, and lungs. Benzene produces damage on CPMS depending on the exposure time and dose. The CPMS technique could be used as an important aromatic hydrocarbons intoxication indicator.
Databáze: MEDLINE
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