Cell Swelling Induced by the Antimalarial KAE609 (Cipargamin) and Other PfATP4-Associated Antimalarials.
| Autor: | Dennis ASM; Research School of Biology, Australian National University, Canberra, Australia., Lehane AM; Research School of Biology, Australian National University, Canberra, Australia., Ridgway MC; Research School of Biology, Australian National University, Canberra, Australia., Holleran JP; Eskitis Institute for Drug Discovery, Griffith University, Brisbane, Australia., Kirk K; Research School of Biology, Australian National University, Canberra, Australia kiaran.kirk@anu.edu.au. |
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| Jazyk: | angličtina |
| Zdroj: | Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2018 May 25; Vol. 62 (6). Date of Electronic Publication: 2018 May 25 (Print Publication: 2018). |
| DOI: | 10.1128/AAC.00087-18 |
| Abstrakt: | For an increasing number of antimalarial agents identified in high-throughput phenotypic screens, there is evidence that they target PfATP4, a putative Na + efflux transporter on the plasma membrane of the human malaria parasite Plasmodium falciparum For several such "PfATP4-associated" compounds, it has been noted that their addition to parasitized erythrocytes results in cell swelling. Here we show that six structurally diverse PfATP4-associated compounds, including the clinical candidate KAE609 (cipargamin), induce swelling of both isolated blood-stage parasites and intact parasitized erythrocytes. The swelling of isolated parasites is dependent on the presence of Na + in the external environment and may be attributed to the osmotic consequences of Na + uptake. The swelling of the parasitized erythrocyte results in an increase in its osmotic fragility. Countering cell swelling by increasing the osmolarity of the extracellular medium reduces the antiplasmodial efficacy of PfATP4-associated compounds, consistent with cell swelling playing a role in the antimalarial activity of this class of compounds. (Copyright © 2018 American Society for Microbiology.) |
| Databáze: | MEDLINE |
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