Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

Autor:	Bergiers I; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Andrews T; Wellcome Trust Sanger Institute, Hinxton, United Kingdom., Vargel Bölükbaşı Ö; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Buness A; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Janosz E; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Lopez-Anguita N; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Ganter K; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Kosim K; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Celen C; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Itır Perçin G; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy., Collier P; Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany., Baying B; Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany., Benes V; Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany., Hemberg M; Wellcome Trust Sanger Institute, Hinxton, United Kingdom., Lancrin C; European Molecular Biology Laboratory, EMBL Rome, Monterotondo, Italy.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2018 Mar 20; Vol. 7. Date of Electronic Publication: 2018 Mar 20.
DOI:	10.7554/eLife.29312
Abstrakt:	Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell transcriptome assays. We found that a heptad of transcription factors (Runx1, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2) is specifically co-expressed in an intermediate population expressing both endothelial and hematopoietic markers. Within the heptad, we identified two sets of factors of opposing functions: one (Erg/Fli1) promoting the endothelial cell fate, the other (Runx1/Gata2) promoting the hematopoietic fate. Surprisingly, our data suggest that even though Fli1 initially supports the endothelial cell fate, it acquires a pro-hematopoietic role when co-expressed with Runx1. This work demonstrates the power of single-cell RNA-sequencing for characterizing complex transcription factor dynamics. Competing Interests: IB, TA, ÖV, AB, EJ, NL, KG, KK, CC, GI, PC, BB, VB, MH, CL No competing interests declared (© 2018, Bergiers et al.)
Databáze:	MEDLINE
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