| Autor: |
Orazbekov Y; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.; South-Kazakhstan State Pharmaceutical Academy, Al-Farabi Square, Shymkent 160019, Kazakhstan., Ibrahim MA; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.; Department of Chemistry of Natural Compounds, National Research Center, Dokki, Cairo 12622, Egypt., Mombekov S; Kazakh National Medical University, Almaty 050000, Kazakhstan., Srivedavyasasri R; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA., Datkhayev U; Kazakh National Medical University, Almaty 050000, Kazakhstan., Makhatov B; South-Kazakhstan State Pharmaceutical Academy, Al-Farabi Square, Shymkent 160019, Kazakhstan., Chaurasiya ND; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA., Tekwani BL; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.; Department of Biomolecular Science, University of Mississippi, University, MS 38677, USA., Ross SA; National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.; Department of Biomolecular Science, University of Mississippi, University, MS 38677, USA. |
| Abstrakt: |
Phytochemical analysis of the ethanolic extract of Maclura pomifera fruits yielded four new compounds ( I - IV ) along with eleven known compounds ( V - XV ). The crude extract exhibited significant activity towards cannabinoid receptors (CB1: 103.4% displacement; CB2: 68.8% displacement) and possibly allosteric interaction with δ and μ opioid receptors (-49.7 and -53.8% displacement, resp.). Compound I was found to be possibly allosteric for κ and μ opioid receptors (-88.4 and -27.2% displacement, resp.) and showed moderate activity (60.5% displacement) towards CB1 receptor. Compound II exhibited moderate activity towards cannabinoid receptors CB1 and CB2 (47.9 and 42.3% displacement, resp.). The known compounds ( V - VIII ) exhibited prominent activity towards cannabinoid receptors: pomiferin ( V ) (IC 50 of 2.110 and 1.318 μ M for CB1 and CB2, resp.), auriculasin ( VI ) (IC 50 of 8.923 μ M for CB1), warangalone ( VII ) (IC 50 of 1.670 and 4.438 μ M for CB1 and CB2, resp.), and osajin ( VIII ) (IC 50 of 3.859 and 7.646 μ M for CB1 and CB2, resp.). The isolated compounds were also tested for inhibition of human monoamine oxidase-A and monoamine oxidase-B enzymes activities, where all the tested compounds showed fewer inhibitory effects on MAO-A compared to MAO-B activities: auriculasin ( VI ) (IC 50 of 1.91 and 45.98 μ M for MAO-B and MAO-A, resp.). |
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