| Autor: |
Khan M; Institute of Chemical Sciences , University of Peshawar , 25120 Peshawar , Pakistan., Koivisto JT; BioMediTech Institute and Faculty of Medicine and Life Sciences , University of Tampere , Lääkärinkatu 1 , 33520 Tampere , Finland., Hukka TI, Hokka M, Kellomäki M; BioMediTech Institute and Faculty of Medicine and Life Sciences , University of Tampere , Lääkärinkatu 1 , 33520 Tampere , Finland. |
| Jazyk: |
angličtina |
| Zdroj: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2018 Apr 11; Vol. 10 (14), pp. 11950-11960. Date of Electronic Publication: 2018 Apr 02. |
| DOI: |
10.1021/acsami.8b01351 |
| Abstrakt: |
Biopolymers are attractive candidates to fabricate biocompatible hydrogels, but the low water solubility of most of them at physiological pH has hindered their applications. To prepare a water-soluble derivative of chitosan (WSC) biopolymer, it was grafted with a small anionic amino acid, l-glutamic acid, using a single-step 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide coupling reaction. This resulted in a zwitterion-tethered structure onto the polymer backbone. The degree of substitution range was 13-16 ± 1.25%, which was controlled by varying the feeding reagent ratios. Differential scanning calorimetry- and X-ray diffraction-based analysis confirmed a transition from amorphous into a moderately amorphous/crystalline morphology after amino acid grafting, which made the derivative water-soluble at physiological pH. Composite hydrogels gelated within 60 s when using this WSC together with benzaldehyde-terminated 4-arm poly(ethylene glycol) as cross-linker. The compressive modulus of these hydrogels could be easily tuned between 4.0 ± 1.0 and 31 ± 2.5 kPa, either by changing the cross-linker concentration or total solid content in the final gel. The gels were injectable at the lowest cross-linker as well as total solid content, due to the enhanced elastic behavior. These hydrogels showed biodegradability during a 1 month incubation period in phosphate-buffered saline with weight remaining of 60 ± 1.5 and 44 ± 1.45% at pHs 7.4 and 6.5, respectively. The cytocompatibility of the gels was tested using the fibroblast cell line (i.e., WI-38), which showed good cell viability on the gel surface. Therefore, these hydrogels could be an important injectable biomaterial for delivery purpose in the future. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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