Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies.

Autor: Edington CD; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Chen WLK; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Geishecker E; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Kassis T; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA., Soenksen LR; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA., Bhushan BM; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA., Freake D; Continuum LLC, Boston, MA, USA., Kirschner J; Continuum LLC, Boston, MA, USA., Maass C; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Tsamandouras N; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Valdez J; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Cook CD; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Gynepathology Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Parent T; Continuum LLC, Boston, MA, USA., Snyder S; Continuum LLC, Boston, MA, USA., Yu J; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Suter E; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Shockley M; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Velazquez J; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Velazquez JJ; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Stockdale L; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Papps JP; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Gynepathology Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Lee I; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Vann N; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Gamboa M; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., LaBarge ME; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Zhong Z; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Wang X; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Boyer LA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Lauffenburger DA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Gynepathology Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Carrier RL; Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Communal C; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Tannenbaum SR; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Stokes CL; Stokes Consulting, Redwood City, CA, USA., Hughes DJ; CnBio Innovations, Hertfordshire, United Kingdom., Rohatgi G; Continuum LLC, Boston, MA, USA., Trumper DL; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. trumper@mit.edu.; Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA, USA. trumper@mit.edu., Cirit M; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. mcirit@mit.edu.; Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. mcirit@mit.edu., Griffith LG; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. griff@mit.edu.; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. griff@mit.edu.; Center for Gynepathology Research, Massachusetts Institute of Technology, Cambridge, MA, USA. griff@mit.edu.; Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. griff@mit.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Mar 14; Vol. 8 (1), pp. 4530. Date of Electronic Publication: 2018 Mar 14.
DOI: 10.1038/s41598-018-22749-0
Abstrakt: Microphysiological systems (MPSs) are in vitro models that capture facets of in vivo organ function through use of specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-system microfluidic platforms that are compatible with the quantitative study of a range of compounds, including lipophilic drugs. We describe three different platform designs - "4-way", "7-way", and "10-way" - each accommodating a mixing chamber and up to 4, 7, or 10 MPSs. Platforms accommodate multiple different MPS flow configurations, each with internal re-circulation to enhance molecular exchange, and feature on-board pneumatically-driven pumps with independently programmable flow rates to provide precise control over both intra- and inter-MPS flow partitioning and drug distribution. We first developed a 4-MPS system, showing accurate prediction of secreted liver protein distribution and 2-week maintenance of phenotypic markers. We then developed 7-MPS and 10-MPS platforms, demonstrating reliable, robust operation and maintenance of MPS phenotypic function for 3 weeks (7-way) and 4 weeks (10-way) of continuous interaction, as well as PK analysis of diclofenac metabolism. This study illustrates several generalizable design and operational principles for implementing multi-MPS "physiome-on-a-chip" approaches in drug discovery.
Databáze: MEDLINE