Photonic modulation of epidermal growth factor receptor halts receptor activation and cancer cell migration.

Autor: Botelho CM; Centre of Biological Engineering, Universidade do Minho, Braga, Portugal.; Centre of Molecular and Environmental Biology (CBMA), Universidade do Minho, Braga, Portugal., Gonçalves O; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark., Marques R; Centre of Molecular and Environmental Biology (CBMA), Universidade do Minho, Braga, Portugal., Thiagarajan V; International Iberian Nanotechnology Laboratory (INL), P-4715-310 Braga, Portugal.; School of Chemistry, Bharathidasan University, Tiruchirappalli, India., Vorum H; Department of Clinical Medicine, Aalborg University Hospital, Aalborg, Denmark., Gomes AC; Centre of Molecular and Environmental Biology (CBMA), Universidade do Minho, Braga, Portugal.; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark., Neves-Petersen MT; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.; International Iberian Nanotechnology Laboratory (INL), P-4715-310 Braga, Portugal.; Department of Clinical Medicine, Aalborg University Hospital, Aalborg, Denmark.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2018 Sep; Vol. 11 (9), pp. e201700323. Date of Electronic Publication: 2018 Jun 19.
DOI: 10.1002/jbio.201700323
Abstrakt: Epidermal growth factor receptor (EGFR) plays a key role in regulating cell survival, proliferation and migration, and its overexpression and activation has been correlated with cancer progression. Cancer therapies targeting EGFR have been applied in the clinic with some success. We show, by confocal microscopy analysis, that illumination of adenocarcinomic human alveolar basal epithelial cells (Human A549-EGFR biosensor cell line) with 280 nm at irradiance levels up to 20 times weaker than the Ultraviolet B (UVB) solar output for short periods of time (15-45 minutes) prevents epidermal growth factor-mediated activation of EGFR located on the cell membrane, preventing or reducing cellular disaggregation, formation of filopodia and cell migration. This effect of Ultraviolet (UV) light illumination was confirmed further in a functional scratch assay, and shown to be more effective than that of a specific EGFR-signaling inhibitor. This new photonic approach may be applicable to the treatment of various types of cancer, alone or in combination with other therapies.
(© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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