Natriuretic peptide system regulation in granulosa cells during follicle deviation and ovulation in cattle.

Autor: De Cesaro MP; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil.; Faculty of Veterinary Medicine, Meridional Institute (IMED), Passo Fundo, Brazil., Dos Santos JT; Instituto Federal de Educação, Ciência e Tecnologia Farroupilha, Campus Frederico Westphalen, Brazil., Ferst JG; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Nóbrega JE Jr; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Rosa P; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Rovani MT; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Ilha GF; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Bohrer RC; Department of Animal Science, McGill University, Sainte Anne de Bellevue, QC, Canada., Ferreira R; Department of Animal Science, Santa Catarina State University, Chapecó, Brazil., Gasperin BG; Department of Animal Pathology, Federal University of Pelotas, Capão do Leão, Brazil., Bordignon V; Department of Animal Science, McGill University, Sainte Anne de Bellevue, QC, Canada., Gonçalves P; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2018 Jun; Vol. 53 (3), pp. 710-717. Date of Electronic Publication: 2018 Mar 14.
DOI: 10.1111/rda.13161
Abstrakt: Natriuretic peptides (NPs) are known to regulate reproductive events in polyovulatory species, but their function and regulation in monovulatory species remain to be fully characterized. Using a well-established in vivo model, we found that bovine granulosa cells from follicles near the deviation stage express mRNA for the three NP receptors (NPR1, NPR2 and NPR3), but not for NP precursors (NPPA, NPPB and NPPC). The abundance of NPR3 mRNA was higher in dominant compared to subordinate follicles at the expected time of follicular deviation. After deviation, mRNA for all NP receptors was significantly more abundant in the dominant follicle. Intrafollicular inhibition of oestrogen receptors downregulated NPR1 mRNA in dominant follicles. In granulosa cells from preovulatory follicles, NPPC mRNA increased at 3 and 6 h after systemic GnRH treatment, but decreased at 12 and 24 h to similar levels observed in samples collected at 0 h. After GnRH treatment, NPR1 mRNA was upregulated at 24 h, NPR3 mRNA gradually decreased after 3 h, while NPR2 mRNA was not regulated. The mRNA expression of the enzyme FURIN increased at 24 h after GnRH treatment. These findings revealed that the expression of mRNA encoding important components of the NP system is regulated in bovine granulosa cells during follicular deviation and in response to GnRH treatment, which suggests a role of NP system in the modulation of these processes in monovulatory species.
(© 2018 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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