Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont.

Autor:	Pluvinage B; Department of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC, V8W 3P6, Canada., Grondin JM; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada., Amundsen C; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada., Klassen L; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada., Moote PE; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada., Xiao Y; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA., Thomas D; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada., Pudlo NA; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA., Anele A; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada., Martens EC; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA., Inglis GD; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada.; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada., Uwiera RER; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada., Boraston AB; Department of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC, V8W 3P6, Canada. boraston@uvic.ca., Abbott DW; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, Canada. wade.abbott@agr.gc.ca.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2018 Mar 13; Vol. 9 (1), pp. 1043. Date of Electronic Publication: 2018 Mar 13.
DOI:	10.1038/s41467-018-03366-x
Abstrakt:	In red algae, the most abundant principal cell wall polysaccharides are mixed galactan agars, of which agarose is a common component. While bioconversion of agarose is predominantly catalyzed by bacteria that live in the oceans, agarases have been discovered in microorganisms that inhabit diverse terrestrial ecosystems, including human intestines. Here we comprehensively define the structure-function relationship of the agarolytic pathway from the human intestinal bacterium Bacteroides uniformis (Bu) NP1. Using recombinant agarases from Bu NP1 to completely depolymerize agarose, we demonstrate that a non-agarolytic Bu strain can grow on GAL released from agarose. This relationship underscores that rare nutrient utilization by intestinal bacteria is facilitated by the acquisition of highly specific enzymes that unlock inaccessible carbohydrate resources contained within unusual polysaccharides. Intriguingly, the agarolytic pathway is differentially distributed throughout geographically distinct human microbiomes, reflecting a complex historical context for agarose consumption by human beings.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu