| Autor: |
Savinova OS; Bach Institute of Biochemistry, Biotechnology Research Center, Russian Academy of Sciences, Moscow, 119071, Russia. savinova_os@rambler.ru., Solyev PN, Vasina DV, Tyazhelova TV, Fedorova TV, Savinova TS |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2018 Jan; Vol. 83 (1), pp. 26-31. |
| DOI: |
10.1134/S0006297918010030 |
| Abstrakt: |
The ability of the ascomycete Aspergillus niger N402 to transform exogenous progesterone was investigated. We found that this strain has steroid-hydroxylating activity and can introduce a hydroxyl group into the progesterone molecule mainly at positions C11(α) and C21 with predominant formation of 21-hydroxyprogesterone (deoxycortone). In addition, formation of 6β,11α-dihydroxyprogesterone was also observed. Studying the effects of the growth medium composition and temperature on progesterone conversion by A. niger N402 showed that the most intense accumulation of 21-hydroxyprogesterone occurred in minimal synthetic medium at 28°C. Increasing the cultivation temperature to 37°C resulted in almost complete inhibition of the hydroxylase activity in the minimal medium. In the complete medium, a similar increase in temperature inhibited 11α-hydroxylase activity and completely suppressed 6β-hydroxylase activity, but it produced no effect on 21-hydroxylating activity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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