Transcriptome analysis of pig intestinal cell monolayers infected with Cryptosporidium parvum asexual stages.

Autor: Mirhashemi ME; Clinical and Translational Institute, Sackler School of Biomedical Sciences, Tufts University, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA.; Cummings School of Veterinary Medicine at Tufts University, Building 20, 200 Westborough Avenue, North Grafton, Massachusetts, 01536, USA., Noubary F; Clinical and Translational Institute, Sackler School of Biomedical Sciences, Tufts University, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA., Chapman-Bonofiglio S; Cummings School of Veterinary Medicine at Tufts University, Building 20, 200 Westborough Avenue, North Grafton, Massachusetts, 01536, USA., Tzipori S; Cummings School of Veterinary Medicine at Tufts University, Building 20, 200 Westborough Avenue, North Grafton, Massachusetts, 01536, USA., Huggins GS; Clinical and Translational Institute, Sackler School of Biomedical Sciences, Tufts University, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA., Widmer G; Cummings School of Veterinary Medicine at Tufts University, Building 20, 200 Westborough Avenue, North Grafton, Massachusetts, 01536, USA. giovanni.widmer@tufts.edu.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2018 Mar 12; Vol. 11 (1), pp. 176. Date of Electronic Publication: 2018 Mar 12.
DOI: 10.1186/s13071-018-2754-3
Abstrakt: Background: Human cryptosporidiosis is caused primarily by two species of apicomplexan protozoa, Cryptosporidium parvum and C. hominis. In cultured cell monolayers, the parasite undergoes two generations of asexual multiplication (merogony). However, the proportion of parasites completing the life-cycle is low and insufficient to sustain continuous propagation. Due to the intracellular location of meronts and later life-cycle stages, oocyst and sporozoites are the only forms of the parasite that can readily be isolated.
Results: Research on the replicating forms of Cryptosporidium parasites and their interaction with the host cell remains challenging. Based on an RNA-Seq analysis of monolayers of pig epithelial cells infected with C. parvum, here we report on the impact of merogony on the host's gene regulation. Analysis of the transcriptome of infected and uninfected monolayers demonstrates a significant impact of the infection on host cell gene expression. A total of 813 genes were differentially expressed. Functional terms significantly altered in response to infection include phosphoprotein, RNA binding and acetylation. Upregulation of cell cycle pathways indicates an increase in mitosis. Notably absent from differentially enriched functional categories are stress- and apoptosis-related functions. The comparison of the combined host-parasite transcriptome reveals that C. parvum gene expression is less diverse than the host cell transcriptome and is highly enriched for genes encoding ribosomal functions, such as ribosomal proteins.
Conclusions: These results indicate that C. parvum infection significantly changes host biological functions and provide new insight into gene functions driving early C. parvum intracellular development.
Databáze: MEDLINE
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