Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

Autor: Keller KE; Oregon Health and Science University, United States., Bhattacharya SK; University of Miami, United States., Borrás T; University of North Carolina-Chapel Hill, United States., Brunner TM; Glaucoma Research Foundation, United States., Chansangpetch S; University of California, San Francisco, United States., Clark AF; University of North Texas Health Sciences Center, United States., Dismuke WM; Aerie Pharmaceuticals, United States., Du Y; University of Pittsburgh, United States., Elliott MH; University of Oklahoma, United States., Ethier CR; Georgia Institute of Technology, United States., Faralli JA; University of Wisconsin, United States., Freddo TF; Massachusetts College of Pharmacy and Health Sciences, United States., Fuchshofer R; University of Regensburg, Germany., Giovingo M; John H. Stroger, Jr. Hospital of Cook County, United States., Gong H; Boston University, United States., Gonzalez P; Duke University, United States., Huang A; University of California, Los Angeles, United States., Johnstone MA; University of Washington, United States., Kaufman PL; University of Wisconsin, United States., Kelley MJ; Oregon Health and Science University, United States., Knepper PA; University of Illinois at Chicago, United States., Kopczynski CC; Aerie Pharmaceuticals, United States., Kuchtey JG; Vanderbilt University, United States., Kuchtey RW; Vanderbilt University, United States., Kuehn MH; University of Iowa, United States., Lieberman RL; Georgia Institute of Technology, United States., Lin SC; University of California, San Francisco, United States., Liton P; Duke University, United States., Liu Y; Augusta University, United States., Lütjen-Drecoll E; University of Erlangen-Nuremberg, Germany., Mao W; University of North Texas Health Sciences Center, United States., Masis-Solano M; University of California, San Francisco, United States., McDonnell F; Duke University, United States., McDowell CM; University of North Texas Health Sciences Center, United States., Overby DR; Imperial College, London, United Kingdom., Pattabiraman PP; Case Western Reserve University, United States., Raghunathan VK; University of Houston, United States., Rao PV; Duke University, United States., Rhee DJ; Case Western Reserve University, United States., Chowdhury UR; Mayo Clinic, United States., Russell P; University of California, Davis, United States., Samples JR; Western Glaucoma Foundation, United States., Schwartz D; EyeSonix, United States., Stubbs EB; Loyola University, Chicago, United States., Tamm ER; University of Regensburg, Germany., Tan JC; University of Southern California, United States., Toris CB; Case Western Reserve University, United States., Torrejon KY; Glauconix, United States., Vranka JA; Oregon Health and Science University, United States., Wirtz MK; Oregon Health and Science University, United States., Yorio T; University of North Texas Health Sciences Center, United States., Zhang J; University of California, Los Angeles, United States., Zode GS; University of North Texas Health Sciences Center, United States., Fautsch MP; Department of Ophthalmology, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, United States. Electronic address: fautsch.michael@mayo.edu., Peters DM; Department of Pathology & Laboratory Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, United States. Electronic address: dmpeter2@wisc.edu., Acott TS; Department of Ophthalmology, Department of Biochemistry & Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, United States. Electronic address: acott@ohsu.edu., Stamer WD; Department of Ophthalmology, Duke University, DUMC 3802, Durham, NC 27705, United States. Electronic address: dan.stamer@duke.edu.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2018 Jun; Vol. 171, pp. 164-173. Date of Electronic Publication: 2018 Mar 09.
DOI: 10.1016/j.exer.2018.03.001
Abstrakt: Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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