Detection of Fusarium verticillioides by PCR-ELISA based on FUM21 gene.

Autor: Omori AM; Department of Pathological Sciences, Center of Biological Sciences, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil., Ono EYS; Department of Biotechnology and Biochemistry, Center of Exact Sciences, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil., Bordini JG; Department of Biotechnology and Biochemistry, Center of Exact Sciences, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil., Hirozawa MT; Department of Biotechnology and Biochemistry, Center of Exact Sciences, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil., Fungaro MHP; Department of General Biology, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil., Ono MA; Department of Pathological Sciences, Center of Biological Sciences, State University of Londrina (UEL), CEP 86057-970, Londrina, Paraná, Brazil. Electronic address: marioono@uel.br.
Jazyk: angličtina
Zdroj: Food microbiology [Food Microbiol] 2018 Aug; Vol. 73, pp. 160-167. Date of Electronic Publication: 2018 Jan 19.
DOI: 10.1016/j.fm.2018.01.020
Abstrakt: Fusarium verticillioides is a primary corn pathogen and fumonisin producer which is associated with toxic effects in humans and animals. The traditional methods for detection of fungal contamination based on morphological characteristics are time-consuming and show low sensitivity and specificity. Therefore, the objective of this study was to develop a PCR-ELISA based on the FUM21 gene for F. verticillioides detection. The DNA of the F. verticillioides, Fusarium sp., Aspergillus sp. and Penicillium sp. isolates was analyzed by conventional PCR and PCR-ELISA to determine the specificity. The PCR-ELISA was specific to F. verticillioides isolates, showed a 2.5 pg detection limit and was 100-fold more sensitive than conventional PCR. In corn samples inoculated with F. verticillioides conidia, the detection limit of the PCR-ELISA was 1 × 10 4 conidia/g and was also 100-fold more sensitive than conventional PCR. Naturally contaminated corn samples were analyzed by PCR-ELISA based on the FUM21 gene and PCR-ELISA absorbance values correlated positively (p < 0.05) with Fusarium sp. counts (CFU/g). These results suggest that the PCR-ELISA developed in this study can be useful for F. verticillioides detection in corn samples.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: