Circulating pathogen-specific plasmablasts in female patients with upper genital tract infection.

Autor: Palkola NV; Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland., Pakkanen SH; Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland., Heikinheimo O; Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Kantele JM; Occupational Health and Environmental Medicine, University of Turku, Turku, Finland., Kantele A; Inflammation Center, Clinic of Infectious Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Unit of Infectious Diseases, Department of Medicine/Solna, Karolinska Institutet, Stockholm, Sweden. Electronic address: anu.kantele@hus.fi.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2018 Apr; Vol. 126, pp. 69-75. Date of Electronic Publication: 2018 Mar 02.
DOI: 10.1016/j.jri.2018.02.005
Abstrakt: Mucosal antibodies constitute the first line of adaptive immune defence against invaders in the female genital tract (FGT), yet the sequence of events leading to their production is surprisingly poorly characterized. We explored the induction of pathogen-specific antibody-secreting cells (ASC) as a response to an acute infection in the upper FGT. We recruited 12 patients undergoing surgery due to an upper FGT infection (7/12 blood culture positive, 5/12 negative) and six healthy controls. Pathogens were sampled during surgery and PBMC collected in the acute phase of the disease (days 7-10). We searched by ELISPOT circulating pathogen-specific ASC and explored their frequency, immunoglobulin isotype distribution, and expressions of homing receptors (α 4 β 7, L-selectin, and CLA). All patients had circulating ASC specific to the infective bacteria; the geometric mean was 434 (95%CI 155-1234) ASC (IgA + IgG + IgM)/10 6 PBMC. IgA ASC predominated in 7/12, IgG ASC in 3/12, and IgM ASC in 2/12 cases. Of all the pathogen-specific ASC, 60% expressed α 4 β 7 , 67% L-selectin, and 9% CLA. This study is the first to show induction of pathogen-specific ASC in the peripheral blood in bacterial infection in the human FGT. Our findings reveal that such FGT-originating pathogen-specific ASC are predominated by IgA ASC and exhibit a homing receptor profile resembling that of ASC in acute urinary tract infection. The data thus suggest a characteristic profile shared by the urogenital tract.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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