Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons.

Autor: Perissinotti PP; Departamento de Fisiología, Facultad de Ciencias Exactas y Naturales, Biología Molecular y Celular 'Dr. Héctor Maldonado', Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Ciudad Autónoma de Buenos Aires, Argentina., Rivero-Echeto MC; Departamento de Fisiología, Facultad de Ciencias Exactas y Naturales, Biología Molecular y Celular 'Dr. Héctor Maldonado', Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Ciudad Autónoma de Buenos Aires, Argentina., Garcia-Rill E; Department of Neurobiology and Developmental Sciences, Center for Translational Neuroscience, University of Arkansas for Medical Sciences, Little Rock, AR, USA., Bisagno V; Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina.; Instituto de Investigaciones Farmacológicas (ININFA), CONICET-Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina., Urbano FJ; Departamento de Fisiología, Facultad de Ciencias Exactas y Naturales, Biología Molecular y Celular 'Dr. Héctor Maldonado', Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina. fjurbano@fbmc.fcen.uba.ar.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Ciudad Autónoma de Buenos Aires, Argentina. fjurbano@fbmc.fcen.uba.ar.
Jazyk: angličtina
Zdroj: Brain structure & function [Brain Struct Funct] 2018 Jun; Vol. 223 (5), pp. 2499-2514. Date of Electronic Publication: 2018 Mar 08.
DOI: 10.1007/s00429-018-1645-x
Abstrakt: Leptin is an adipose-derived hormone that controls appetite and energy expenditure. Leptin receptors are expressed on extra-hypothalamic ventrobasal (VB) and reticular thalamic (RTN) nuclei from embryonic stages. Here, we studied the effects of pressure-puff, local application of leptin on both synaptic transmission and action potential properties of thalamic neurons in thalamocortical slices. We used whole-cell patch-clamp recordings of thalamocortical VB neurons from wild-type (WT) and leptin-deficient obese (ob/ob) mice. We observed differences in VB neurons action potentials and synaptic currents kinetics when comparing WT vs. ob/ob. Leptin reduced GABA release onto VB neurons throughout the activation of a JAK2-dependent pathway, without affecting excitatory glutamate transmission. We observed a rapid and reversible reduction by leptin of the number of action potentials of VB neurons via the activation of large conductance Ca 2+ -dependent potassium channels. These leptin effects were observed in thalamocortical slices from up to 5-week-old WT but not in leptin-deficient obese mice. Results described here suggest the existence of a leptin-mediated trophic modulation of thalamocortical excitability during postnatal development. These findings could contribute to a better understanding of leptin within the thalamocortical system and sleep deficits in obesity.
Databáze: MEDLINE
Externí odkaz: