| Autor: |
Netala VR; a Department of Biotechnology , Sri Venkateswara University , Tirupati , India., Bukke S; b Department of Biochemistry , Sri Venkateswara University , Tirupati , India., Domdi L; a Department of Biotechnology , Sri Venkateswara University , Tirupati , India., Soneya S; a Department of Biotechnology , Sri Venkateswara University , Tirupati , India., G Reddy S; a Department of Biotechnology , Sri Venkateswara University , Tirupati , India., Bethu MS; c Division of Biology , Indian Institute of Chemical Technology , Hyderabad , India., Kotakdi VS; d DST-PURSE Centre , Sri Venkateswara University , Tirupati , India., Saritha KV; a Department of Biotechnology , Sri Venkateswara University , Tirupati , India., Tartte V; e Department of Botany , Sri Venkateswara University , Tirupati , India. |
| Abstrakt: |
The present study reports the biosynthesis of silver nanoparticles (IH-AgNPs) using aqueous leaf extract of Indigofera hisruta L. The biosynthesized IH-AgNPs were found to be FCC crystals, 5-10 nm in size, spherical in shape and stable. The biosynthesized IH-AgNPs showed dose-dependant cytotoxicity against prostate cancer (PC3) (IC 50 = 68.5 μg/mL), colon cancer (COLO205) (IC 50 = 85.2 μg/mL), and mouse melanoma (B16F10) (IC 50 = 80.9 μg/mL). IH-AgNPs were found to be nontoxic towards normal CHO (Chinese hamster ovary) cells. The biosynthesized IH-AgNPs showed effective in vitro antioxidant activity against DPPH (IC 50 = 63.43 μg/mL) and H 2 O 2 (IC 50 = 89.93 μg/mL) radicals. IH-AgNPs exhibited effective antibacterial activity against both Gram+ve and Gram-ve bacteria. MIC values of IH-AgNPs against S. aureus, B. subtilis, P. aeruginosa and E. coli were found to be 7.8 μg/mL, 3.9 μg/mL, 15.6 μg/mL and 15.6 μg/mL respectively. IH-AgNPs also showed inhibitory activity against fungal pathogens including C. albicans, C. nonalbicans and C. tropicalis. Considering the results together, we demonstrate that IH-AgNPs exhibits three different bioactivities (3-in-1 system) and they could be employed as future antimicrobial, antioxidant and anticancer agents/drug delivery vehicles in the field of biomedicine. |
