| Autor: |
DeAtley KL; Department of Animal and Range Sciences , New Mexico State University , Las Cruces , New Mexico 88003 , United States., Colgrave ML; CSIRO, Agriculture and Food , 306 Carmody Road , St. Lucia , Queensland 4067 , Australia., Cánovas A; Centre for Genetic Improvement of Livestock, Department of Animal Biosciences , University of Guelph , Guelph , Ontario N1G 2W1 , Canada., Wijffels G; CSIRO, Agriculture and Food , 306 Carmody Road , St. Lucia , Queensland 4067 , Australia., Ashley RL; Department of Animal and Range Sciences , New Mexico State University , Las Cruces , New Mexico 88003 , United States., Silver GA; Department of Animal and Range Sciences , New Mexico State University , Las Cruces , New Mexico 88003 , United States., Rincon G; Zoetis Animal Health , Kalamazoo , Michigan 49007 , United States., Medrano JF; Department of Animal Science , University of California , Davis , California 95616 , United States., Islas-Trejo A; Department of Animal Science , University of California , Davis , California 95616 , United States., Fortes MRS; School of Chemistry and Molecular Biosciences , University of Queensland , St. Lucia , Queensland 4042 , Australia.; Queensland Alliance for Agriculture and Food Innovation, St. Lucia , Queensland 4072 , Australia., Reverter A; CSIRO, Agriculture and Food , 306 Carmody Road , St. Lucia , Queensland 4067 , Australia., Porto-Neto L; CSIRO, Agriculture and Food , 306 Carmody Road , St. Lucia , Queensland 4067 , Australia., Lehnert SA; CSIRO, Agriculture and Food , 306 Carmody Road , St. Lucia , Queensland 4067 , Australia., Thomas MG; Department of Animal Sciences , Colorado State University , Fort Collins , Colorado 80523 , United States. |
| Abstrakt: |
Puberty in cattle is regulated by an endocrine axis, which includes a complex milieu of neuropeptides in the hypothalamus and pituitary gland. The neuropeptidome of hypothalamic-pituitary gland tissue of pre- (PRE) and postpubertal (POST) Bos indicus-influenced heifers was characterized, followed by quantitative analysis of 51 fertility-related neuropeptides in these tissues. Comparison of peptide abundances with gene expression levels allowed assessment of post-transcriptional peptide processing. On the basis of classical cleavage, 124 mature neuropeptides from 35 precursor proteins were detected in hypothalamus and pituitary gland tissues of three PRE and three POST Brangus heifers. An additional 19 peptides (cerebellins, PEN peptides) previously reported as neuropeptides that did not follow classical cleavage were also identified. In the pre-pubertal hypothalamus, a greater diversity of neuropeptides (25.8%) was identified relative to post-pubertal heifers, while in the pituitary gland, 38.6% more neuropeptides were detected in the post-pubertal heifers. Neuro-tissues of PRE and POST heifers revealed abundance differences ( p < 0.05) in peptides from protein precursors involved in packaging and processing (e.g., the granin family and ProSAAS) or neuron stimulation (PENK, CART, POMC, cerebellins). On their own, the transcriptome data of the precursor genes could not predict the neuropeptide profile in the exact same tissues in several cases. This provides further evidence of the importance of differential processing of the neuropeptide precursors in the pituitary before and after puberty. |
