Enhanced cholinergic-tone during the stress induce a depressive-like state in mice.

Autor: Fernandes SS; Post-Graduation Program in Health Sciences, Faculty of Medicine, Laboratory of Neurosciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Koth AP; Post-Graduation Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Parfitt GM; Post-Graduation Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Cordeiro MF; Post-Graduation Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Peixoto CS; Post-Graduation Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Soubhia A; Post-Graduation Program in Health Sciences, Faculty of Medicine, Laboratory of Neurosciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil., Moreira FP; Translational Science on Brain Disorders, Clinical Neuroscience Lab., Department of Health and Behavior, Catholic University of Pelotas (UCPel), Pelotas, RS, Brazil., Wiener CD; Post-Graduation Program in Epidemiology, Federal University of Pelotas (UFPel), Pelotas, RS, Brazil., Oses JP; Translational Science on Brain Disorders, Clinical Neuroscience Lab., Department of Health and Behavior, Catholic University of Pelotas (UCPel), Pelotas, RS, Brazil., Kaszubowski E; Department of Psychology, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil., Barros DM; Post-Graduation Program in Health Sciences, Faculty of Medicine, Laboratory of Neurosciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil; Post-Graduation Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil; Institute of Biological Sciences, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. Electronic address: barrosdm@yahoo.com.br.
Jazyk: angličtina
Zdroj: Behavioural brain research [Behav Brain Res] 2018 Jul 16; Vol. 347, pp. 17-25. Date of Electronic Publication: 2018 Mar 01.
DOI: 10.1016/j.bbr.2018.02.044
Abstrakt: Major depressive disorder has a heterogeneous etiology, since it arises from the interaction of multiple factors and different pathophysiological mechanisms are involved in the symptomatology. This study aimed to investigate the role of the cholinergic system in the susceptibility to stress and, consequently, in the depression-like behavior. C57BL/6 mice were treated with Physostigmine (PHYS), an acetylcholinesterase (AChE) inhibitor, and were submitted to the social defeat stress. For the behavioral evaluation of the locomotor activity, anxiety-like and depression-like behaviors the open field, elevated plus maze, sucrose preference, social interaction and forced swim were used. Hippocampus and prefrontal cortex samples were collected for evaluation of AChE activity, as well as blood samples for analysis of serum cortisol levels. Our results showed that 15 min after the injection of PHYS there was a significant inhibition of AChE activity in the hippocampus and in the prefrontal cortex. On the other hand, in the end of the experimental design, day 12, there was no difference in AChE activity levels. Inhibition of AChE and exposure to the stress led to an increase in cortisol levels. Animals that received PHYS and were exposed to stress showed less social interaction and greater learned helplessness, anhedonia and anxious-like behavior. Taken together, our findings suggest that increasing the cholinergic tone shortly before stress induction impacts on the ability to cope with upcoming stressful situations, leading to a depressive-like state.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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