Time does matter! Acute copper exposure abolishes rhythmicity of clock gene in Danio rerio.

Autor: Doria HB; Federal University of Paraná (UFPR), Department of Cellular Biology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil. Electronic address: halinadoria@gmail.com., Ferreira MB; Federal University of Paraná (UFPR), Department of Cellular Biology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil., Rodrigues SD; Federal University of Paraná (UFPR), Department of Basic Pathology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil., Lo SM; Federal University of Paraná (UFPR), Department of Basic Pathology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil., Domingues CE; Ponta Grossa State University (UEPG), Research Group on Environmental and Sanitary Analytical Chemistry (QAAS), Caixa Postal 992, Postal Code: 84030-900, Ponta Grossa, Paraná, Brazil., Nakao LS; Federal University of Paraná (UFPR), Department of Basic Pathology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil., de Campos SX; Ponta Grossa State University (UEPG), Research Group on Environmental and Sanitary Analytical Chemistry (QAAS), Caixa Postal 992, Postal Code: 84030-900, Ponta Grossa, Paraná, Brazil., Ribeiro CAO; Federal University of Paraná (UFPR), Department of Cellular Biology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil., Randi MAF; Federal University of Paraná (UFPR), Department of Cellular Biology, School of Biological Sciences, Caixa Postal 19.031, Postal Code: 81531-990, Curitiba, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2018 Jul 15; Vol. 155, pp. 26-36. Date of Electronic Publication: 2018 Feb 28.
DOI: 10.1016/j.ecoenv.2018.02.068
Abstrakt: The circadian clock is a key cellular timing system that coordinates physiology and behavior. Light is a key regulator of the clock mechanism via its activation of Per and Cry clock gene expression. Evidence points to a key role of reactive oxygen species (ROS) in resetting this process. In this context, the aim of the present study was to explore copper as a ROS generator, using an innovative approach investigating its effects on circadian timing. Liver and brain from Danio rerio specimens exposed to 0, 5, 25 and 45 μg/L copper concentrations were obtained. Daily oscillations of superoxide dismutase (SOD) and catalase (CAT) enzymatic activity and their correlations both with clock genes (per1, per2, and cry1a) and with organism energy cost were determined. CAT expression correlates with per2 and cry1a and, thus, provides data to support the hypothesis of hydrogen peroxide production by a phototransducing flavin-containing oxidase. Higher SOD activity is correlated with higher intracellular ATP levels. Copper disturbed the daily oscillation of antioxidant enzymes and clock genes, with disturbed per1 rhythmicity in both the brain and liver, while cry1a rhythmicity was abolished in the liver at 25 μg/L copper. Coordination between the SOD and the CAT enzymes was lost when copper concentrations exceeded the limits established by international laws. These results indicate that organism synchronization with the environment may be impaired due to acute copper exposure.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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