| Autor: |
Oliveira ICF; a Post-Graduate Program in Pharmaceutical Sciences, Pharmacy and Veterinary Medicine Graduate Courses , University of Sorocaba , Sorocaba , Brazil., Mora AD; a Post-Graduate Program in Pharmaceutical Sciences, Pharmacy and Veterinary Medicine Graduate Courses , University of Sorocaba , Sorocaba , Brazil., Neto JB; b Post-Graduate Program in Technological and Environmental Processes and Computer Engineering Graduate Course , University of Sorocaba , Sorocaba , Brazil., de Oliveira ÁL; a Post-Graduate Program in Pharmaceutical Sciences, Pharmacy and Veterinary Medicine Graduate Courses , University of Sorocaba , Sorocaba , Brazil., Harder C; c University Paulista , São Paulo , Brazil., Hanai-Yoshida VM; b Post-Graduate Program in Technological and Environmental Processes and Computer Engineering Graduate Course , University of Sorocaba , Sorocaba , Brazil., Cogo JC; d Post-Graduate Programs in Biomedical Engineering and Bioengineering , University Brazil , São Paulo , Brazil., Floriano RS; e Department of Pharmacology , State University of Campinas , Campinas , Brazil., Bonventi Junior W; b Post-Graduate Program in Technological and Environmental Processes and Computer Engineering Graduate Course , University of Sorocaba , Sorocaba , Brazil., Oliveira SJ; a Post-Graduate Program in Pharmaceutical Sciences, Pharmacy and Veterinary Medicine Graduate Courses , University of Sorocaba , Sorocaba , Brazil., Oshima-Franco Y; a Post-Graduate Program in Pharmaceutical Sciences, Pharmacy and Veterinary Medicine Graduate Courses , University of Sorocaba , Sorocaba , Brazil. |
| Abstrakt: |
Bothrops jararacussu venom's (Bj2015) batch was biomonitored quarterly for one year to assess phospholipase A 2 (PLA 2 ) activity, immunogenicity, neurotoxicity, and myotoxicity. In silico models were applied to evaluate losses using decay model and recoveries by predictive trend analysis. Mice were immunized with Bj2015. Antibodies were detected by double-immunodiffusion and total protein and albumin were measured. Neuromuscular blockade-induced by 40 μg mL -1 venom solution was carried out using mouse nerve phrenic-diaphragm preparation. Resulting muscles were submitted to light microscopy to evaluate the myotoxicity. PLA 2 activity of 0.1 mg mL -1 Bj2015 was measured using 4-nitro-3-(octanoyloxy)benzoic acid as substrate. Over time, greater losses occurred in neurotoxicity than PLA 2 , but not in myotoxicity and immunogenicity. Concluding, the neurotoxicity decrease can be related to enzymatic losses, including PLA 2 . Depending on the purpose of use, the collected venom responds on a long time, avoiding unnecessary new collections, improving life quality of animals in captivity and increasing their longevity. |
