Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India.

Autor:	Prabhu KN; Commonwealth Veterinary Association-Crucell Rabies Diagnostic Laboratory, Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. nithinprabhuk@yahoo.com.; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. nithinprabhuk@yahoo.com., Isloor S; Commonwealth Veterinary Association-Crucell Rabies Diagnostic Laboratory, Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. kisloor@gmail.com.; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. kisloor@gmail.com., Veeresh BH; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. veereshhanchinal@gmail.com., Rathnamma D; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. doddamanerathnamma@yahoo.co.in., Sharada R; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. sharadadr@yahoo.co.in., Das LJ; Department of Microbiology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. lekshmijdas10@gmail.com., Satyanarayana ML; Department of Pathology, Veterinary College-Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru 560024, India. mlspathology@yahoo.com., Hegde NR; National Institute of Animal Biotechnology, Miyapur, Hyderabad 500049, India. hegde@niab.org.in., Rahman SA; Commonwealth Veterinary Association, Jayanagar, Bengaluru 560011, India. shireencva@gmail.com.
Jazyk:	angličtina
Zdroj:	Veterinary sciences [Vet Sci] 2018 Feb 28; Vol. 5 (1). Date of Electronic Publication: 2018 Feb 28.
DOI:	10.3390/vetsci5010024
Abstrakt:	Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well as the more sensitive, reverse transcription polymerase chain reaction (RT-PCR), have recently been employed for the laboratory diagnosis of rabies. We compared the three methods on brain samples from domestic (dog, cat, cattle, buffalo, horse, pig and goat) and wild (leopard, wolf and jackal) animals from various parts of India. Of the 257 samples tested, 167 were positive by all the three tests; in addition, 35 of the 36 decomposed samples were positive by RT-PCR. This is the first study in which such large number of animal samples have been subjected to the three tests simultaneously. The results confirm 100% corroboration between DFA and dRIT, buttress the applicability of dRIT in the simple and rapid diagnosis of rabies in animals, and reaffirm the suitability of RT-PCR for samples unfit for testing either by DFA or dRIT. Competing Interests: The authors declare no conflicts of interest.
Databáze:	MEDLINE
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