Standardization of a latex agglutination test for coproantigen detection of Fasciola sp. in bovine cattle stool.

Autor: Orejarena Ávila LP; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia., Inguilan Benavides EM; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia., Padilla Sanabria L; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia., Recalde-Reyes DP; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia., Rodríguez-Salazar CA; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia., Castaño-Osorio JC; 1Grupo Inmunología Molecular (Gymol), Universidad del Quindío, Armenia, Quindío Colombia.; 2Facultad Ciencias de la Salud, Universidad del Quindío, Carrera 15 Calle 12 Norte, Armenia, Quindío Colombia.
Jazyk: angličtina
Zdroj: Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2018 Mar; Vol. 42 (1), pp. 55-61. Date of Electronic Publication: 2017 Nov 10.
DOI: 10.1007/s12639-017-0964-9
Abstrakt: Fasciolosis is a zoonotic parasitic disease, which affects humans and animals; diagnosed through noncommercial immunoassay tests that cannot be used on the field. Thereby, establishing the optimal conditions to develop a latex agglutination technique with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. is a priority. Latex particles were sensitized with IgG and IgM antibodies directed against excretion/secretion antigens of Fasciola sp. The specificity of the antibodies was determined against antigens of different helminths and protozoa; the sensitivity and specificity of the test was evaluated against a previously standardized direct ELISA. The coupling rates of the IgG and IgM antibodies were 85.77 and 100%, respectively. The minimum detectable concentration of Fasciola sp. excretion/secretion antigens, diluted in a phosphate-buffered saline, was 1.589 mg/mL(IgG) and 0.158 mg/mL(IgM) and for the antigens incorporated in the bovine cattle stool it was 3.178 mg/mL(IgG) and 1.589 mg/mL(IgM). The test showed crossed reaction against Giardia sp., and Cryptosporidium sp. antigens. Agreement of the IgG and IgM latex test against the ELISA test was of 78.78 and 96.96%, respectively; the specificity found was of 100% for both tests and sensitivity was 78.79% (IgG) and 96.97% (IgM). This work standardized the latex agglutination technique to detect Fasciola sp. antigens in bovine cattle stool.
Competing Interests: Compliance with ethical standardsThe authors, herein, declare no conflict of interest.During the process to obtain serum protein containing PAb IgM, the animal was maintained in good hygienic conditions and fed constantly; trained staff performed the inoculation process and sacrifice to minimize the risk of death or suffering during the procedure. This project used the least amount of reagents to avoid large quantities of chemical and biological wastes. All the biological material in the laboratory was treated with adequate biosecurity standards, thus, preventing contamination of the area. The project was fully carried out in the Biomedical Research Center, CIBM, at Universidad del Quindío, adhering to protocols and management under the responsibility of the researchers and the Molecular Immunology Group “GYMOL”.
Databáze: MEDLINE
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