The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile.

Autor: Thieme S; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany., Holzbaur A; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany., Wiedemuth R; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany., Binner A; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany., Navratiel K; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany., Anastassiadis K; Stem Cell Engineering, BIOTEC, Technische Universitaet Dresden, Dresden, Germany., Brenner S; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany.; Center for Regenerative Therapies Dresden, Technische Universitaet Dresden, Dresden, Germany., Richter C; Department of Pediatrics, University Clinic 'Carl Gustav Carus' Dresden, Dresden, Germany.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Feb 28; Vol. 13 (2), pp. e0192437. Date of Electronic Publication: 2018 Feb 28 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0192437
Abstrakt: Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
Databáze: MEDLINE
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