Adapted MS/MS ALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species.

Autor: Gao F; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., McDaniel J; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Chen EY; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Rockwell HE; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Nguyen C; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Lynes MD; Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA., Tseng YH; Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA.; Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA., Sarangarajan R; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Narain NR; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA., Kiebish MA; BERG, LLC, 500 Old Connecticut Path, Bldg B, 3rd Floor, Framingham, MA 01701, USA.
Jazyk: angličtina
Zdroj: Lipids [Lipids] 2018 Jan; Vol. 53 (1), pp. 133-142.
DOI: 10.1002/lipd.12004
Abstrakt: Cardiolipin (Ptd 2 Gro) is a complex, doubly charged phospholipid located in the inner mitochondrial membrane where it plays an essential role in regulating bioenergetics. Abnormalities in Ptd 2 Gro content or composition have been associated with mitochondrial dysfunction in a variety of disease states. Here, we report the development of an adapted high-resolution data-independent acquisition (DIA) MS/MS ALL shotgun lipidomic method to enhance the accuracy and reproducibility of Ptd 2 Gro molecular species quantitation from biological samples. Utilizing the doubly charged molecular ions and the isotopic pattern with negative mode electrospray ionization mass spectrometry (ESI-MS) using an adapted MS/MS ALL approach, we profiled more than 150 individual Ptd 2 Gro species, including monolysocardiolipin (MLPtd 2 Gro). The method described in this study demonstrated high reproducibility, sensitivity, and throughput with a wide dynamic range. This high-resolution MS/MS ALL shotgun lipidomics approach could be extended to screening aberrations of Ptd 2 Gro metabolism involved in mitochondrial dysfunction in various pathological conditions and diseases.
(© 2018 AOCS.)
Databáze: MEDLINE
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